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Test Code =Lact Ven Lactic Acid Blood, Venous Syringe

Important Note

Place specimen in outside pocket of a pre-filled biohazard bag of ice, then put into a separate biohazard bag for transport.

For regional sites, please refer to Lactic Acid Blood, Venous in LabMan for directions on how to collect and process samples for lactate testing on the chemistry analyzer.

Clinical System Name

Lactic Acid Blood, Venous syringe

Synonyms

Lactate, Venous syringe

=lactate

Description

The concentration of lactate in the blood is dependent on the rate of production in muscle cells and erythrocytes and the rate of metabolism in the liver.  Lactic acidosis usually results from overproduction or underutilization of lactate.  Elevated lactate levels can occur as a result of tissue hypoxia; diabetes mellitus; phenformin therapy; malignancies; glycogen storage disease; ethanol, methanol or salicylate ingestion; and metabolic acidosis.      

Sample Requirements

Specimen:  Whole Blood

Container(s): Heparinized Syringe

Required Vol: 2 mL

Minimum Vol: 0.5mL

 

Notes: Place specimen in separate bag and then in bag with ice. Remove air bubbles from Heparinized Syringe  immediately after collection. A venous sample is preferable unless an arterial line is in place.

For hemodilution patients, note the collection site. Method is affected by exposure to RBC's (longer than 15 minutes), drawing blood with stasis, and by oxygen depletion (exercise, hypoxia, crying). Do not use tourniquet for more than 30 seconds at a time and do not allow patient to flex or clench fist. 

Sample volume can be shared with venous blood gas, ionized calcium, =glu and =lytes

 

Processing Instructions

Reject due to:  age of specimen, wrong specimen container, hemolysis

Spin: Y

Aliquot: Y

Temp: 2-8oC

Storage location: Core Chemistry Refrigerator

 

Off-site collection: Testing is only available at Main Campus. For regional sites, please refer to Lactic Acid Blood, Venous in LabMan for directions on how to collect and process samples for lactate testing on the chemistry analyzer.

Stability

 

 

Specimen Type Temperature Time
Whole Blood (Syringe) RT < 15 M
Whole Blood (Syringe)  0-4oC

30 min

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617

 

 

Reference Range

0.5 - 2.2 mMol/L

Methodology

Method:  amperometric method by  SIEMENS RAPIDPOINT 500

Analytical Volume: 0.2 mL

CPT Codes

83605