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Test Code 1,25 VIT D 1,25-Dihydroxyvitamin D, Serum

Important Note

This test is privliged to Seattle Children's Endocrinologists and Nephrologists, and can be ordered directly from select Cerner powerplans.  All other providers should order a Miscellaneous Test Communication.

Reporting Name

1,25-Dihydroxyvitamin D, S

Useful For

As a second-order test in the assessment of vitamin D status, especially in patients with renal disease

 

Investigation of some patients with clinical evidence of vitamin D deficiency (eg, vitamin D-dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxy vitamin D)

 

Differential diagnosis of hypercalcemia

Seattle Children's Hospital Note:

Synonyms:

Renal Failure

Bone Disease


Specimen Required


Patient Preparation: Fasting (4-hour preferred but not required)

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: At least 1.5 mL


Seattle Children's Hospital Note:

Collect  AT LEAST 2 mL WHOLE BLOOD in a red or gold top tube.

Specimen Type

Serum

Specimen Minimum Volume

0.7 mL

Seattle Children's Hospital Note:

Serum

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  28 days
  Ambient  7 days

Day(s) and Time(s) Performed

Monday through Friday; 3 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Extraction/Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Method Description

Deuterated stable isotopes (d3-1,25 dihydroxyvitamin D3 [DHVD3-d3] and d6-1,25 dihydroxyvitamin D2 [DHVD2-d6]) are added to a 1-mL plasma sample as internal standard. 1,25-Dihydroxyvitamin D2 (DHVD2), 1,25-dihydroxyvitamin D3 (DHVD3), and the internal standard are extracted using acetonitrile precipitation. The extracts are then further purified by SPE and affinity extraction. Extracts are then derivatized using 4-phenyl-1, 2,4-triazoline-3,5-dione and analyzed by chromatography-tandem mass spectrometry using multiple reaction monitoring with a C18 turbo cleanup for excess derivatizing reagent. DHVD2 and DHVD3 are quantified and a total is reported with a clinical reference range attached to the total DHVD.(Unpublished Mayo method)

Reference Values

Males

<16 years: 24-86 pg/mL

≥16 years: 18-64 pg/mL

Females

<16 years: 24-86 pg/mL

≥16 years: 18-78 pg/mL

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

82652

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DHVD 1,25-Dihydroxyvitamin D, S 62290-2

 

Result ID Test Result Name Result LOINC Value
8822 1,25-Dihydroxyvitamin D, S 62290-2

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Seattle Children's Hospital Additional Information:

Send-out Instructions: Batch and send out Mon - Fri with the Mayo courier.