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T&B Lymphocyte Studies

Important Note

Note: limited specimen stability - see Sample Requirements below - do NOT refrigerate.

Children's Outpatient Blood Draw areas and Community Services - order "T&B Community Services" (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.

For more information, call Cell Markers Lab at (206) 987-2560.

Additional Codes

MULTIPLE ORDER SELECTIONS

Ordering Providers: select desired tests on requisition or in CIS - see table below.  For outside requisitions, please be sure to check box for Lymphocyte Subset Analysis AND select appropriate panel in lower box. 

Clinical System Name Panel Information
T and B CD3, CD4 Lymphocyte Subsets - CD3, CD4
T and B CD3 and Subsets Lymphocyte Subsets - CD3, CD4, CD8
T and B CD3, CD4, ATG Monitoring Lymphocyte Subsets - CD3, CD4 ATG Monitoring
T and B CD3, CD4, B Cells Lymphocyte Subsets - CD3, CD4, CD19, CD20
T and B CD3, Subsets, B Cells Lymphocyte Subsets - CD3, CD4, CD8, CD19, CD20
T and B CD3, Subsets, B Cells, NK Cells Lymphocyte Subsets - CD3, CD4, CD8, CD16, CD19, CD20, CD56 (Formerly SCCA panel)
T and B Full Panel with Natural Killer Cells Lymphocyte Subsets - Full Panel (Includes: CD2, CD3, CD4, CD8, CD16, CD19, CD56, HLA DR) (Formerly Full Panel - other immunodeficiencies)
T and B Full Panel with Natural Killer Cells and TCR Lymphocyte Subsets - Full Panel with TCR (Includes: CD2, CD3, CD4, CD8, CD16, CD19, CD56, HLA DR plus TCR alpha beta and gamma delta) (Formerly Full Panel - other immunodeficiencies + TCR)
T and B Full Panel no Natural Killer Cells Lymphocyte Subsets - HIV Panel (Includes: CD2, CD3, CD4, CD8, CD19, HLA DR)

The minimum panel available includes CD4 and CD3. Panels can be ordered alone, or in combination with other T and B Panels - duplicate tests will not be charged.

For TCR, ALPS, CD45 RA RO, Leukocyte Adhesion Workup - see separate listings.

Clinical System Name

Multiple selections - SEE CHART ABOVE

Synonyms

B And T Cells
CD4/8 Ratio
Immunodeficiency Panel
Lymphocyte Subset Analysis
NK Cells
T&B Cells
T B
T Cell Subsets
T&B Lymphocyte Studies
T4/T8 Lymphocyte Subsets
TB 3 sub
TB Comm
TB F NK
TB F NKTCR
TB FnoNK

T and B

.B cell

B cell subsets

Description

This group of assays are used for enumerating the percentages and absolute cell counts for lymphocyte subsets in whole blood.  The tests are used in the evaluation of immune function, in the setting of primary and secondary immune deficiencies, post-transplant, and monitoring monoclonal antibody therapies.

 

Note:  'B cell phenotyping' and 'T cell phenotyping' are separate tests performed in Cell Markers (B cell phenotyping) and sent out to Mayo Medical Laboratories in Rochester (T cell phenotyping) - see separate listings for details

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; Dark Green/Sodium Heparin also acceptable from referring labs if CBC & differential results submitted.  Microtainers not recommended.

Preferred Vol:  2-3 mL

Minimum Vol:  1 mL total in single tube

 

Note:  T&B SHOULD SHARE one lavender tube with CBC Diff (if ordered).  Specimens received after 14:30 will be held and testing will be performed the next working day.

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday collect any time ok if received at SCH main lab same day, if not do not collect
Saturday ok if collected before 12 noon do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

Children's Outpatient Blood Draw areas and Community Services - order "T&B Community Services" (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.

 

Processing Instructions

Reject due to:  clotted specimen, Microtainer, Refrigerated

Spin: N

Aliquot: N

Temp:  RT 

Note:  Have Core Lab run specimen through hematology analyzer and send a copy of the results and requisition with specimen to Cell Markers.

Storage location: Days:  Transport specimen, copy of community services requisition (if applicable), and labels to 10th floor Cell Markers (station #280).    Eves/Nights:  Store specimen, copy of community services requisition (if applicable), and labels in the Cell Markers RT box in CPA.

 

Off-site collection:  Keep whole blood at room temperature. Referring labs may send CBC w/differential results from the same collection along with the sample.

Stability

Temperature Time
Room temp EDTA - 30 hours; Heparin - 48 hours
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
N M-F, S (0730-1200) 2-3 days

 

Note: limited specimen stability - see Sample Requirements for details.

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

Reference Range

Reference values accompany patient report.
 

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on patient's white count and testing requested

Limitations:  refrigeration selectively decreases subsets - refrigerated samples not acceptable.

CPT Codes

LYMPHOCYTE SUBSET ANALYSIS (T&B) PANELS CPT CODE(S)
Full Panel with Natural Killers (CD2, CD3, CD4, CD8, CD16, CD19, CD56, HLA DR)  
     CM  AB IF Total T Cells CD2 86359
     CM  AB IF Total T Cells CD3 86359
     CM AB IF CD4/8 86360
     CD3/19/45 DIR IF Stain/Run/Analyze 86355
     CD3/16/45 DIR IF Stain/Run/Analyze 86357
     CD3/56/45 DIR IF Stain/Run/Analyze 86357
     CM  AB IF HLA-DR 86356
Full Panel without Natural Killers (CD2, CD3, CD4, CD8, CD19, HLADR  
     CM  AB IF Total T Cells CD2 86359
     CM  AB IF Total T Cells CD3 86359
     CM AB IF CD4/8 86360
     CD3/19/45 DIR IF Stain/Run/Analyze 86355
     CM  AB IF HLA-DR 86356
B Cells (CD3, CD4, CD19, CD20)  
     CM  AB IF Total T Cells CD3 86359
     CD3/4/45 DIR IFStain/Run/Analysis 86361
     CD3/19/45 DIR IFStain/Run/Analysis 86355
     CM AB IF Total B Cells CD20 86355
CD3 & Subsets  
     CM  AB IF Total T Cells CD3 86359
     CM AB IF CD4/8 86360
CD3/CD4 only  
     CM  AB IF Total T Cells CD3 86359
     CD3/4/45 DIR IFStain/Run/Analysis 86361
*the minimum panel available includes CD3 & CD4  


For additional information, please contact Laboratory Client Services at 206-987-2617.