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Renal Biopsy, Histo/Path

Important Note

Nephrologist must notify Histology (206) 987-2580 at least 24 hours in advance.  Weekend biopsies should be discussed beforehand with pathologist on call. FRESH (unfixed) sample should be deliver immediately to Histology. 

Clinical System Name

Surgical Pathology Final Report

Synonyms

Kidney Biopsy

Kidney transplant biopsy

Renal transplant biopsy

Description

Needle core biopsy of kidney is procured from native or allograft kidney to assess etiology of altered function. Routine processing and histopathology is always performed with addition of EM, IF, culture, etc. as necessitated by clinical setting.

Sample Requirements

Specimen:  Tissue

Container(s):  Saline-moistened gauze or telfa in sterile specimen container

Preferred size>2.0 cm in total length

Minimum size:  n/a

 

Note:  FRESH (no fixative) kidney biopsy must be delivered immediately to 8th floor lab CPA or Histology (OC.8.901) and given to someone's personal charge; identify it as a fresh specimen.  Specific handling instructions may be obtained from Histology at (206) 987-2580.

 

A completed surgical pathology request form including hospital number, name, date of birth, date of procedure along with a kidney biopsy worksheet must be submitted with the specimen. 

 

After 1700, deliver specimens to the 8th floor lab CPA. 

 

Off-site collection:  Specific handling instructions may be obtained from Histology at (206) 987-2580.  Biopsy should be divided , ensuring glomeruli are present in each piece.  One portion frozen in OCT or submerged in Michel's transport media for IF, one piece diced into glutaraldehyde for EM (which is sent out) & the remainder in formalin for LM.  Send frozen piece on dry ice.  Send EM on wet ice.

 

Processing Instructions

Reject due to: NA

Spin:  N

Aliquot:  N

Temp: RT

Note:   Notify Histology immediately when specimen arrives in Lab.  Contact on-call pathologist (206) 987-2131 if Histology is unavailable.

 

Stability

Specimen Type Temperature Time
Tissue Room temp 10 min
  Refrigerated 30 min
  Frozen N

 

Availability

STAT Performed TAT
  M-F 8 a.m. - 5 p.m. 3 days

 

Performing Laboratory

Seattle Children's Laboratory  

Department

Department:  Histology

Phone Number: (206) 987-2580; for results inquiries call (206) 987-2103.

 

 

Methodology

Method: Varies; routine formalin fixation with paraffin embedding and standard sectioning; possible addition of frozen section immunofluorescence and electron microscopic examination

Analytical Volume:  n/a

CPT Codes

CPT codes will vary depending on tests performed.  Call Client Services for more information (206) 987-2617.

Requisition

Surgical pathology requisition required.

Kidney biopsy worksheet.