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Direct Thrombin Inhibitor 

Clinical System Name

Miscellaneous Test

Synonyms

Argatroban Level

Lepirudin Level

Thrombin Inhibitor-Direct

Bivalirudin level

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol: (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within 1 hour of collection.

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: -70 C

Storage location: Spin blood for 10 minutes; remove plasma. Re-spin plasma for an additional 10 minutes. Transfer to 2 plastic tubes, each affixed with a large computer label and freeze in the -70° C freezer. Process within 2 hours of draw.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

 

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
plasma Room temp 2 h
  Refrigerated  
  Frozen  

 

Availability

STAT Performed TAT
Yes, . Drawn daily, sent M - F 1 - 3 d

 

Performing Laboratory

UWMC

Department

Department:  

UWMC

 

Dept of Laboratory Medicine

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number

 

 

Reference Range

 

16 - 25 s

 

Therapeutic ranges are:

Agatroban: 60-100 seconds

Bivalirudin: 60-90 seconds

Dabigatran: this assay should not be used for Dabigatran monitoring. 

 

 

Methodology

Method: Clot based

Analytical Volume: None specified

Limitations:

CPT Codes

85670

 

Send Out Instructions

 

Reference Test Name: Direct Thrombin Inhibitor 
Reference Test Number: DTI
Instructions: Send Stat frozen on dry ice via Delivery Express. Send routines out Mon - Fri with the UW/HMC courier. Keep a copy of the Batch Sheet for our records.