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CSF Malignancy Workup - Flow Cytometry

Important Note

Specimens for CSF Malignancy Workup must be coordinated with Pathologist/Cell Markers Laboratory prior to collection to ensure testing availability and adequate specimen collection.  Order MISC test (specify: Flow Cytometry) and under special instructions, request that an additional (ideally) 10-20 mL be collected for flow.

 

For further information, contact Hematopathology (Cell Markers) at 206-987-2560 or Pathology 987-2103.

 

Note:  'FH Flow' is only to be used for BMT/HSCT patients.  For all other SCH patients, order MISC test (flow cytometry).

Clinical System Name

Miscellaneous Test

Synonyms

CSF Flow Workup Request
Flow Cytometry Immunophenotyping (Leukemia)
Immunophenotyping
Malignancy, CSF

MRD

Description

CSF Malignancy Workup (by Flow Cytometry) is used in determining the lineage of hematopoietic precursors which may be present in the CSF in acute leukemia.

Sample Requirements

Specimen:  Cerebrospinal Fluid (CSF)

Container(s):  CSF collection tubes

Preferred Vol:  10-20 mL; volume dependent on blast count 

Minimum Vol:  dependent on blast count; consult lab

Note:  Specimens for CSF Malignancy Workup must be coordinated with Pathologist/Cell Markers Laboratory prior to collection to ensure testing availability and adequate specimen collection.

 

For further information, contact Hematopathology (Cell Markers) at 206-987-2560 or Pathology 987-2103. 

 

Note: this test is for CSF workups only - for bone marrow, peripheral blood, or other body fluids, see separate listing(s).  peripop        

 

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location:  Days: M - F until 1630, transport specimen and labels to 10th floor Cell Markers (station #280); call before tubing specimen to make sure there is someone available to do testing.

After hours: Contact pathologist on call.

 

Off-site collection:  not accepted

Stability

Temperature Time
Room temp must be processed immediately
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
Y - with pathologist approval

Monday - Friday 0800-1630;  SEE ORDERING NOTES ABOVE.

2-3 days

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

None specified
 

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on blast count; consult lab

Limitations:  None specified

CPT Codes

contact Laboratory Client Services at (206)-987-2617

Requisition

Order online in CIS -- MISC test.  Specify flow cytometry.