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Test Code A GL BM AB Glomerular Basement Membrane Antibodies

Clinical System Name

Glomerular Basement Membrane Antibodies

Synonyms

Anti GBM

Anti-GBM

Anti-Glomerular Basement Membrane
Antiglomerular Basement Membrane Antibody
GBM
Glomerular Basement Membrane Antibody

Description

This assay is intended for the detection of IgG class antibodies to Glomerular Basement Membrane (GBM) in human serum.  It is to be used as an aid in the diagnosis of various autoimmune vasculitic disorders characterized by elevated levels of selected autoantibodies.  Anti GBM antibodies aid in the diagnosis of Goodpasture's Syndrome.  The reported result is semi-quantitative.

Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST; Red also acceptable

Preferred Vol: 2 mL

Minimum Vol: 1 mL

 

Processing Instructions

Reject due to:

Spin:Y

Aliquot: Y

Temp: 2-8 C

Storage location: CPA refrigerator, FrigF rack. 

 

Off-site collection: Spin blood, refrigerate 1 mL serum in a polypropylene aliquot tube.

Stability

Temperature Time
Room temp

 

Refrigerated 7 d
Frozen

6 m

 

Availability

STAT Performed TAT
N *

M-F

1-3 d

*Available STAT only upon approval of Seattle Children's Hospital Pathologist on call.

Performing Laboratory

Seattle Children's Laboratory

Department

Department:  Chemistry

Location: Chem East

Phone Number: (206) 987-2617

 

 

Reference Range

 

  AI
Negative <1.0
Positive >1.0

 

Methodology

Method: Immunoassay on Bioplex 2200

Analytical Volume: 0.4 mL serum

Limitations:

CPT Codes

83520