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Test Code AG STIM Antigen Stimulation Study

Important Note

Note: limited specimen stability - see Sample Requirements below. 

Ordering Providers:  Please be sure to indicate stimulants requested (Tetanus and/or Candida).

Off-site Collection:  If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning.  See additional notes under 'Processing Instructions - Offsite Collection'.

Clinical System Name

Antigen Stimulation Study Request

Synonyms

Lymphocyte Antigen Stimulation
Lymphocyte Function Analysis
T cell proliferation to antigens
 

Description

The Antigen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to specific stimuli (Tetanus and/or Candida).

Sample Requirements

Specimen:  Whole Blood

Container(s):  Dark Green/Sodium Heparin (no serum separator);

Preferred Vol:  10 mL

Minimum Vol:  10 mL (if unable to collect 10 mL, please contact lab at 206-987-2560; see note above)

 

Note: 

Specimens stable for only 24 hours at room temperature.

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday must be received before 12 noon Friday must be received at SCH main lab before 12 noon Friday
Saturday do not collect do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon day before holiday
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

Call the Cell Marker Lab (206) 987-2560 with any questions regarding specimen collection or handling. If unable to collect minimum volume, please collect as close to 10 mL as possible (10 mL each for Mitogen and Antigen) and notify Cell Markers at 206-987-2560 (leave voicemail after hours).

Draw an additional 10.0 mL if a mitogen (PHA, CD3) is also ordered.   

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location:  Days:  Transport specimen, (copy of community services requisition, if applicable), and labels to 10th floor Cell Markers (station #280).  Notify Cell Markers at x72560 right away if specimen is near 24 hour specimen stability limit - billing/ordering problems should not delay delivery to lab.   Eves/Nights:  Store specimen, (copy of community services requisition, if applicable), and labels in the Cell Markers RT box in CPA.

 

Off-site collection:  Dark Blue/Li Heparin ok.  A normal control should accompany the specimen. It is critical that samples be kept at room temperature; use extra packing to maintain temperature. If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning. Testing must be set up within 24 hours of collection. Draw an additional 10.0 mL if an mitogen (PHA, CD3) is also ordered.  Transport all tubes at RT to Seattle Children's Hospital address on requisition: Laboratory OC.8.720, 4800 Sand Point Way NE, Seattle, WA  98105. 

Stability

Temperature Time
Room temp 24 hours
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
N

Monday - Thurs; Friday must be in lab before noon

7 - 10 days

Limited specimen stability - see specimen requirements.

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

Reference values accompany patient report.

Methodology

Method:  Lymphocyte proliferation with 3H thymidine incorporation

Analytical Volume:  dependent on absolute lymphocyte count

Limitations:

CPT Codes

 

  CPT CODE
Set up and Initial Mitogen or Antigen Stimulant

86353

Each additional Mitogen or Antigen Stimulant 86353