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Test Code ALDOSTERN Aldosterone, Serum

Reporting Name

Aldosterone, S

Useful For

Investigation of primary aldosteronism (eg, adrenal adenoma/carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome)

Specimen Required

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1.2 mL

Collection Instructions: 8 a.m. draw time (after the patient is active for 2 hours) is recommended; preferably no later than 10 a.m.

Additional Information: See Renin-Aldosterone Studies in Special Instructions for more detailed instructions.

Seattle Children's Hospital Note:

Collect 2.4 mL whole blood in a Red top.

Specimen Type


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 30 days
  Refrigerated  28 days
  Ambient  4 days

Day(s) and Time(s) Performed

Monday through Friday; 4 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Method Description

Aldosterone-d6 is added to serum and plasma samples as an internal standard. Aldosterone and aldosterone-d6 are extracted from the specimens using a Strata X cartridge. The eluate is dried down under nitrogen, reconstituted with 70/30 methanol/H2O containing estriol and analyzed by liquid chromatography-tandem mass spectrometry using multiple reaction monitoring in the negative mode.(Fredline VF, Taylor PJ, Dodds HM, Johnson AG: A reference method for the analysis of aldosterone in blood by high-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. Anal Biochem 1997 Oct 15;252(2):308-313)

Reference Values

0-30 days: 17-154 ng/dL*

31 days-11 months: 6.5-86 ng/dL*

1-10 years:

≤40 ng/dL (supine)*

≤124 ng/dL (upright)*

≥11 years: ≤21 ng/dL (a.m. peripheral vein specimen)


*Loeuille GA, Racadot A, Vasseur P, Vandewalle B: Blood and urinary aldosterone levels in normal neonates, infants and children. Pediatrie 1981;36:335-344


For SI unit Reference Values, see

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALDS Aldosterone, S 1763-2


Result ID Test Result Name Result LOINC Value
8557 Aldosterone, S 1763-2

Reject Due To


Mild OK; Gross OK


Mild OK; Gross OK


Mild OK; Gross OK



Testing Algorithm

See Steroid Pathways in Special Instructions.


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

General Request Form (T239) (

Cardiovascular Test Request Form (T724) (