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Test Code ALLPnl UM PhLike B-ALL Fusion Panel

Important Note

This panel includes ABL1 fusion multiplex, JAK fusion multiplex and Kinase fusion multiplex.

Testing is time sensitive due to RNA instability. Recommend collection Monday-Thursday (avoiding holiday weekends), in order to be received within 24 hrs of sample collection.

Synonyms

RT-PCR for ALL,  B-ALL fusion multiplex full panel,  ABL1 fusion multiplex,  JAK fusion multiplex,  Kinase fusion multiplex,  ALL fusion,  B-ALL fusion full panel,  ALL kinase fusion,  RT-PCR kinase fusion detection,  RT-PCR fusion full panel for B-ALL,  Multiplex RT-PCR fusion detection for B-ALL,  B-cell acute lymphoblastic leukemia (B-ALL)

Sample Requirements

Specimen:  Whole Blood or Bone Marrow

Container(s):  Lavender/EDTA (preferred)

Preferred Vol:  4 mL blood; 4 mL bone marrow

Minimum Vol: 

 

Transfer 4 mL bone marrow or whole blood to COG ALL shipping media. If COG ALL shipping media is unavailable, transfer 4 mL bone marrow or whole blood to tissue culture media.

 

Pathology review by a ChildLab pathologist will be performed on submitted samples to verify tumor percentage.

 

A diagnostic or relapse sample previously submitted under a COG protocol can also be used in most cases (some cases may require approval from the study chair); please call the laboratory to confirm sample availability.

 

A minimum of 10% blasts is required to report a result within a mixed cell population. A blast percentage of less than 10% may be reported as unsatisfactory (no results reported)

Processing Instructions

Reject due to: Add-on testing

Spin:  NO

Aliquot:  YES; transfer to COG ALL shipping media

Temp:  REFRIGERATE; DO NOT FREEZE

Storage location:  Store sample in CPA refrigerator sendouts rack. 

 

Testing is time sensitive due to RNA instability. If shipping is delayed, please call the laboratory. Please call (614) 722-2866 prior to shipping and provide tracking number. Ship for overnight delivery. Saturday are deliveries accepted. Please check “Saturday Delivery” on shipment label.

 

Notes: Bone marrow or peripheral blood must be transferred to COG ALL shipping media and refrigerated prior to shipping. COG ALL shipping media is located in Sendouts refrigerator (second shelf, blue rack). Ship at room temperature within 24 hours of collection.

Stability

Specimen Type Temperature Time
  Room temp 1 day
  Refrigerated 4 days
  Frozen NO

 

Availability

STAT Performed TAT
N Mon -Fri. 10 days

 

Performing Laboratory

Nationwide Children's CHILDLAB

Department

Department: 

Nationwide Children's Hospital

ChildLab Central Processing Room C-1961

Attn. Cytogenetics/Molecular Genetics

700 Children's Drive

Columbus, OH 43205

 

Phone Number:  (614) 722-5321

 

 

Reference Range

Interpretive report provided.

 

A minimum of 10% blasts is required to report a result within a mixed cell population. A blast percentage of less than 10% may be reported as unsatisfactory (no results reported).

Methodology

Method:  Reverse transcriptase polymerase chain reaction (RT-PCR). Sanger sequencing

Analytical Volume:  None specified

Limitations:  None specified

Special Instructions

Links to:

 

Requisition

 

Clinical Form

Clinical Utility

Recent studies have identified a subtype of newly diagnosed and relapsed B-progenitor acute lymphoblastic leukemia (B-ALL) children with a gene expression profile suggestive of an alternate kinase fusion in the absence of a classical, t(9;22) BCR-ABL1 rearrangement.

 

Note: Each multiplex assay may be ordered individually if previous testing indicates a targeted approach can be taken.

Send Out Instructions

 

Reference Test Name: B-ALL Fusion Detection Full Panel, Multiplex RT-PCR
Reference Test Number: ALLFUSMA
Instructions:

Ship at ambient temperature within 24 hours of collection. Ship for overnight delivery. Saturday are deliveries accepted. Please check “Saturday Delivery” on shipment label.

 

Testing is time sensitive due to RNA instability. If shipping is delayed, please call the laboratory. Please call (614) 722-2866 prior to shipping and provide tracking number. Ship for overnight delivery. Saturday are deliveries accepted. Please check “Saturday Delivery” on shipment label.