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Test Code ALPS Scr ALPS Screen

Important Note

Note: limited specimen stability - see Sample Requirements below - do NOT refrigerate.

Children's Outpatient Blood Draw areas and Community Services - order "T&B Community Service" (rather than T&B or specific tests which will be ordered in Cell Markers Lab and submit copy of requisition with specimen.

Cell Markers Staff:  In 'DOE' add "T and B CD3, subsets, ALPS Screen" to T&B Comm Service accession number.

For more information call Cell Markers (206) 987-2560.

Additional Codes

Panel includes: ALPS screen (TCR alpha/beta positive, double negative T cells). CD3, CD4, CD8 are run as part of ALPS testing but results are not reported separately. If CD3, CD4, CD8 results are needed, additional order for Lymphocyte Subsets are required. Panels can be ordered alone, or in combination with other T and B Panels - duplicate tests will not be charged.

Clinical System Name

ALPS Screen

Synonyms

Autoimmune Lymphoproliferative Syndrome Screen
T and B CD3, Subsets, ALPS Screen

Description

This assay is used in the evaluation for ALPS (Autoimmune Lymphoproliferative Syndrome).  ALPS is a chronic, lymphoproliferative disorder caused by mutations in the genes that are involved in apoptosis.  The impaired apoptosis causes accumulation of lymphocytes. The lymphoproliferative syndrome reflects the expansion of a normal but typically very low level T-subset that expresses Alpha-Beta TCR but neither CD4 nor CD8 (double negative T cells).

 

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; Dk Green/Heparin also acceptable from referring labs if CBC & differential results submitted

Preferred Vol:  2-3 mL

Minimum Vol:  1 mL; no microtainers

 

Note:  Specimens received after 1430 will be held and testing will be performed the next working day. 

 

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday collect any time ok if received at SCH main lab same day, if not do not collect
Saturday ok if collected before 12 noon do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

Children's Outpatient Blood Draw areas and Community Services - order "T&B Community Services" (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.

Processing Instructions

Reject due to:  clotted specimen, Microtainers, Refrigerated

Spin: N

Aliquot: N

Temp:  RT - DO NOT REFRIGERATE

Note:  Have Core Lab run specimen through hematology analyzer and send a copy of the results and community service requisition (if applicable) with specimen to Cell Markers.

Storage location: Days:  Transport specimen, community service requisition (if applicable), and labels to 10th floor Cell Markers (station #280).    Eves/Nights:  Store specimen, copy of requisition (if applicable), and labels in the Cell Markers RT box in CPA.

 

Off-site collection:  Keep whole blood at room temperature. Referring labs may send CBC w/differential results from the same collection along with the sample.

Stability

Temperature Time
Room temp EDTA - 30 hours; Heparin - 48 hours
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
N M-F, Sa (0730-1200) 2-3 days

Note: limited specimen stability - see Sample Requirements

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

Reference Range

Reference values accompany patient report.
 

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on patient's white count and testing requested

Limitations:

CPT Codes

contact Laboratory Client Services at (206) 987-2617