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Test Code AMOEBA IGG Entamoeba histolytica Antibody, Serum

Reporting Name

E. Histolytica Ab, S

Useful For

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess


Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a Red top.

Short stability, do not draw Wednesday evenings or Thursdays. Any questions call send outs at 987-2563.

Specimen Type


Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 5 days
  Refrigerated  5 days

Day(s) and Time(s) Performed

Tuesday, Thursday; 9 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Method Description

The patient's serum is diluted 1:64 and 100 mcL is placed in microtiter plate wells that have been coated with Entamoeba histolytica HK-9 soluble antigens. After 10 minutes of incubation, the wells are washed and a protein-horseradish peroxidase reagent is added. Color development occurs after addition of peroxide and the tetramethylbenzidine chromogen. The reaction can be read visually or with the aid of a spectrophotometer.(Maddison SE: Serodiagnosis of parasitic diseases. Clin Microbiol Rev 1991;4:457-469)

Reference Values

Expected values: negative

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
SAM E. Histolytica Ab, S 22285-1


Result ID Test Result Name Result LOINC Value
9049 E. Histolytica Ab, S 22285-1

Reject Due To


Mild OK; Gross reject


Mild OK; Gross reject






If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (