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Test Code AMOEBA IGG Entamoeba histolytica Antibody, Serum

Reporting Name

E. histolytica Ab, S

Useful For

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess

 

Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas


Advisory Information


Direct detection of Entamoeba histolytica in stool specimens is recommended to diagnose intestinal amebiasis. See OAP / Parasitic Examination.



Shipping Instructions


 



Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood in a Red top.

Short stability, do not draw Wednesday evenings or Thursdays. Any questions call send outs at 987-2563.

Specimen Type

Serum

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 30 days
  Refrigerated  7 days

Day(s) and Time(s) Performed

Tuesday, Thursday; 9 a.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Method Description

Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled Protein A (the conjugate). The enzyme converts the colourless substrate (urea peroxide/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥620 nm.(Package insert: RIDASCREEN Entamoeba histolytica IgG, R-Biopharm AG, Darmstadt, Germany, 06/10/2016)

Reference Values

Negative

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86753

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SAM E. histolytica Ab, S 22285-1

 

Result ID Test Result Name Result LOINC Value
9049 E. histolytica Ab, S 22285-1

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)