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Test Code AMYL Amylase Serum

Important Note

Specimen must be serum; DO NOT use green top tubes.

Clinical System Name

Amylase Serum


Amylase is an amylolytic digestive enzyme produced by the exocrine pancreas and salivary glands. Amylase is increased in acute pancreatitis, pancreatic abscess or pseudocyst, pancreatic trauma, amyloidosis, pancreatic neoplasm, common-bile-duct obstruction, and after thoracic surgery.  Increased amylase activity may be found in mumps parotitis and renal insufficiency.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Gold Microtainer, Red

Preferred Vol: 0.5 mL

Minimum Vol:  0.5 mL


Note:  Test is affected by lipemia, oxylate, fluoride, citrate, and heparin.  Test is not affected by hemolysis and icterus.  Do not freeze samples.

Processing Instructions

Reject due to: Wrong collection container, frozen specimen

Spin: Y

Aliquot: Y

Temp: 2-4o C

Storage location:  CORE 5 Refrigerator



Off-site collection:  Specimen must be serum; DO NOT use green top tubes.  Spin blood, refrigerate serum. Avoid freezing.


Specimen Type Temperature Time
Serum RT

≤ 7 D

Serum 2-4oC

≤ 30 D





STAT Performed TAT
Y 24/7 1 H


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Lab Chemistry

Phone Number: 206-987-2617



Reference Range


All Ages

≤ 115 IU/L at 37o C



Method: Spectrophotometric, Enzyme Rate by Vitros 4600

Minimum Volume: 0.05 mL serum

Analytical Volume:  0.02 mL + 0.03 mL dead space

CPT Codes