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Test Code ANCA Anti Neutrophil Cytoplasmic AB (ANCA)

Important Note

Other Assays with Similar Test Name

Anti Neutrophil Antibody by Flow Cytometry >4 months old - see separate listing

Anti Neutrophil Antibody by Flow Cytometry <4 months old - see separate listing

Anti Nuclear Antibody (ANA) - see separate listing

Additional Codes

All ANCA serum is saved for 6 months. If MPO/PR3 testing is desired, order testing as an Add-on Communication in CIS or call laboratory (206) 987-2102.

Clinical System Name

Anti Neutrophil Cytoplasmic Antibody

Synonyms

P-ANCA

C-ANCA

Perinuclear ANCA

Cytoplasmic ANCA

Description

Anti-neutrophil cytoplasmic antibody (ANCA) testing is used in the diagnosis and evaluation of various forms of autoimmune mediated vasculitis and microscopic polyangitis.  May be useful for monitoring treatment response in patients with Granulomatosis with Polyangitis [Wegener's Granulomatosis].

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST; Red also acceptable

Preferred Vol:  2 mL

Minimum Vol:  0.6 mL or 2 full Gold Microtainers

 

Note: 

Grossly lipemic and hemolyzed specimen are unacceptable (mild ok).  Plasma samples not accepted.

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  Y

Aliquot:  Y

Temp:  -20 C or colder

Storage location:  Cell Markers box in CPA freezer.  Transfer serum to a plastic aliquot tube.

 

Off-site collection:  Avoid hemolysis. Spin sample, freeze serum in plastic aliquot container.

Stability

Temperature Time
Room temp 4 hours
Refrigerated 1 week
Frozen 6 months

 

Availability

STAT Performed TAT
N Drawn daily; testing performed in batches within 7-10 days

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

Result and interpretation will be provided.
 

Methodology

Method:  Immunofluorescent Assay

Analytical Volume:  100 microL serum

Limitations:  Grossly lipemic and hemolyzed specimens are unacceptable.

CPT Codes

 

  CPT CODE(S)
ANCA

86255

ANCA TITER (reflex) 86256

This test has two components: ANCA screen and ANCA titer (automatic when ANCA screen is positive).