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Test Code ANEUT<4MO Anti Neutrophil Antibody by Flow Cytometry <4 months old

Important Note

ORDERING PROVIDERS

This test needs to be scheduled with the Cell Markers Lab at 206-987-2560 prior to specimen collection.  Both the mother and father need to be drawn, in addition to the patient.

This test is for the detection of allo antibodies in patients <4 months of age.

 

CHILDREN'S OUTPATIENT BLOOD DRAW AREAS AND COMM SERVICE ORDERING

Specimen Collection at Seattle Childrens' Outpatient Blood Draw:  [ANEUT<4 MO] test is ordered on child only.  Both the mother and father need to be drawn in addition to their child.  Have each parent register for the day and use their separate accounts to order the appropriate test, either [ANEUT DAD] or [ANEUT MOM].  Label each of the three sets of samples with the correct Pathnet label (child, mother, or father) and submit to CPA.  Note: if child is INPATIENT, parents may be drawn separately at Outpatient Blood Draw, but sample collection needs to be coordinated so testing can occur together.

Community Services Ordering:  [ANEUT<4 MO] test is ordered on child only.  Both the mother and father samples are required, but for Community Services samples collected elsewhere, register the child only.  Note: if specimens on parents not received, CONTACT CELL MARKERS.  Label child sample with Pathnet label [ANEUT<4MO]; parent tubes should arrive labeled with the parent name and DOB; no additional labeling is necessary.  Notify Cell Markers at x72560 right away - billing/ordering questions should not delay delivery to lab.  

 

Other Assays with Similar Test Name

Anti Neutrophil Antibody by Flow Cytometry >4 months old - see separate listing

Anti Neutrophil Cytoplasmic Antibody (ANCA) - see separate listing

Anti Nuclear Antibody (ANA) - see separate listing

Clinical System Name

Anti Neutrophil AB by Flow <4 months old

Synonyms

Anti Neutrophil Antibodies - Allo

Neutrophil Alloantibodies

Neutrophil Antibodies - Allo

Anti Neutrophil AB by Flow <4 months old

Description

Anti-neutrophil antibody <4 months testing is used to identify the presence of anti-neutrophil antibodies in patients who are <4 months of age, with suspected allo-immune neutropenia.

Sample Requirements

Specimen:  See Notes below

Container(s):  See Notes below

Preferred Vol:  See Notes below

Minimum Vol:  See Notes below

Notes:  Specimens from Child, Mother, and Father are required for patient <4 months, and all samples must be tested together.

 

  Specimen Requirements
CHILD 1 - 2 mL in Gold SST
MOTHER 5 mL in Lavender/EDTA and 2 mL in Gold SST
FATHER 5 mL in Lavender/EDTA

 

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  see Notes below

Aliquot:  see Notes below

Temp:  see Notes below

Storage location:  Notify Cell Markers at x72560 right away - billing/ordering questions should not delay delivery to lab.  Transport specimens, (copy of community services requisition, if applicable), and labels to 10th floor Cell Markers (station #280). Eves/nights:  Store serum in Cell Markers box in CPA freezer; store EDTA specimens, (copy of community services requisition, if applicable), and labels in the Cell Markers RT box in CPA.

 

Notes:  Do not spin Lavender/EDTA tubes (mother and father) and keep at RT; spin Gold SST tubes (child and mother) and transport at RT (after hours, see note above).

 

Off-site collection:  Specimen collection at Seattle Children's preferred - otherwise specimens must be received before noon at Seattle Children's Lab M-F to allow testing within 24 hours of draw.  Both the mother and father need to be drawn in addition to the child and must be tested together (see detailed requirements in Sample Requirements table above).  Label each of the three sets of samples with the correct name and DOB.  Record names & DOB for mother and father, as requested, on child's requisition form. 

Notes:  Do not spin Lavender/EDTA tubes (mother and father) and keep at RT; spin Gold SST tubes (child and mother).  Transport all tubes at RT to Seattle Children's Hospital address on requisition: Laboratory OC.8.720., 4800 Sand Point Way NE, Seattle, WA  98105.  Notify Cell Markers Lab at (206)987-2560.

Stability

Temperature Time
Room temp 24 hours; see Processing below
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
Y processed before noon M-F; no weekends or holidays 1 day

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

Negative

 

Methodology

Method:  Flow Cytometry

Analytical Volume:  see notes

Limitations:

CPT Codes

contact Laboratory Client Services at (206)-987-2617

Clinical Utility

Alloimmune neutropenia, found in patients less than 4 months of age, is seen when the patient's neutrophils are destroyed by maternally-derived alloantibodies.  These antibodies arise in the mother as the direct result of a neutrophil incompatibility between the mother and the fetus, as when an HNA-1b negative mother carries an HNA-1b positive fetus.  The mother becomes sensitized to HNA-1b positive neutrophils when variable amounts of fetal cells pass through the placental barrier and gain entrance into the maternal circulation.  The presence of these "foreign" fetal neutrophils initiates the formation of anti HNA-1b antibodies in the mother, which are then capable of crossing the placenta and causing neutropenia in the infant during the fetal and/or neonatal period.

 

The anti-neutrophil alloantibodies destroy neutrophils which may lead to neutropenia in the infant and subsequent increased risk of infection.

 

Alloimmune neutropenia usually resolves on its own as the levels of maternal antibody naturally decrease.  Alloimmune antibodies are typically gone by 4 months of age.

 

Identification of anti-neutrophl antibodies can help to exclude other inherited forms of neutropenia.