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Test Code ANEUT>4MO Anti Neutrophil Antibody by Flow Cytometry >4 months old

Important Note

Other Assays with Similar Test Name

Anti Neutrophil Antibody by Flow Cytometry <4 months old - see separate listing

Anti Neutrophil Cytoplasmic Antibody (ANCA) - see separate listing

Anti Nuclear Antibody (ANA) - see separate listing

Clinical System Name

Anti Neutrophil AB by Flow >4 months old


Anti Neutrophil Antibodies - Auto

Neutrophil Autoantibodies

Neutrophil Antibodies - Auto

Anti Neutrophil AB by Flow >4 months old


Antineutrophil antibody >4 months testing is used to identify the presence of anti-neutrophil antibodies in patients with suspected autoimmune neutropenia.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST; Red also acceptable

Preferred Vol:  2 mL

Minimum Vol:  2 Gold Microtainers


Note:  Parents' blood not required for this assay.  Draws may be done at any time.


Processing Instructions

Reject due to:  n/a - send to lab

Spin:  Y

Aliquot:  Y

Temp:  2-5 degrees, Refrigerator

Storage location: Cell Markers box in CPA refrigerator.  Refrigerate in plastic aliquot container and include labels and a copy of requisition. 


Off-site collection:  Spin sample, refrigerate serum in plastic aliquot container.


Temperature Time
Room temp 4 hours
Refrigerated 1 week
Frozen 6 months



STAT Performed TAT
N Processed daily; testing performed in batches < 2 weeks


Performing Laboratory

Seattle Children's Laboratory    


Department:  Cell Markers

Phone Number: 206-987-2560



Reference Range




Method:  Flow Cytometry

Analytical Volume:  100 microL serum


CPT Codes





Clinical Utility

Autoimmune neutropenia is seen when a person's immune system produces autoantibodies directed against the neutrophilic proteins on neutrophils.


The anti-neutrophil autoantibodies destroy neutrophils leading to neutropenia and subsequent increased risk for infection.


Autoimmune neutropenia typically spontaneously resolves within 2 years.


Identification of anti-neutrophil autoantibodies can help to exclude other inherited forms of neutropenia.