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Test Code ANTI PR3 Anti Proteinase 3 Level

Clinical System Name

Anti Proteinase 3 Level

Synonyms

ANCA C
ANCA-C
Anti-Proteinase 3 (C-ANCA)
C ANCA
C-ANCA
 

Description

This assay is intended for the detection of IgG class antibodies to PR3 (Proteinase 3) in human serum. It is used as an aid in the diagnosis of various autoimmune vasculitic disorders characterized by elevated levels of anti-neutrophil cytoplasmic antibodies (ANCA), especially microscopic polyangitis (MPA).  May also be useful to monitor treatment response in MPA.  The reported result is semi-quantitative.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST; Red also acceptable

Preferred Vol:  2 mL

Minimum Vol:  1 mL

Processing Instructions

Reject due to

Spin:  Y

Aliquot:  Y

Temp:  2-8C

Storage location:  CPA refrigerator, FrigF rack. 

 

Off-site collection:  Avoid hemolysis. Spin blood, refrigerate 1 mL serum in a polypropylene aliquot tube.

Stability

Temperature Time
Room temp  
Refrigerated 7 d
Frozen 6 m

 

Availability

STAT Performed TAT
N* M-F 1-3 d

 

*Available STAT only upon approval of Seattle Children's Hospital Pathologist on call.

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Chemistry

Location: Chem East

Phone Number: (206) 987-2617

 

 

Reference Range

  AI
Negative < 0.4
Equivocal 0.4 - 0.9
Positive > 1.0

 

Methodology

Method:  Immunoassay on Bioplex 2200

Analytical Volume:  0.4 mL serum

Limitations:

CPT Codes

CPT CODE(S)
86021