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Test Code APCR Activated Protein C Resistance

Important Note

This assay can be used during heparin or oral anticoagulant therapy, and is not sensitive to lupus inhibitors.

Clinical System Name

Activated Protein C Resistance

Synonyms

Activated Protein C Cofactor
APC Cofactor Ration
APC Cofactor Screen
APC Resistance
 

Sample Requirements

Specimen: Whole Blood

Container(s):Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol:  (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within 1 hour of collection.

Processing Instructions

Reject due to:  Clotted, Hemolyzed, Insufficient quantity, and  Improper collection.

Spin: N

Aliquot: N

Temp: RT

Storage location: Core 6 Freezer  ( -70 C) Send Out rack.

 

Note:  Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within  1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots  0.6 mL, affix large label and store in -70 freezer, Send Out rack. Store remaining labels and copy of requisition in the clear plastic box on the CPA fridge.

 

 

Off-site collection:  Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N Drawn daily; Set-up M & Th at HMC Coag Lab 1 - 5 d

 

Performing Laboratory

University of Washington

Dept. of Laboratory Medicine

Harborview Medical Center Coag Lab

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department:  Send Outs

 

Phone Number: (206) 987-2563

 

Reference Range

 

Negative

 

 

Methodology

Method: Clot based

Analytical Volume: 1.0 mL citrate plasma

Limitations:

CPT Codes

85307

 

Send Out Instructions

 

Reference Test Name: Activated Protein C Resistance
Reference Test Number: APCR
Instructions: Send out M - F with the UW courier.