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Test Code BCR/ABL p190 BCR/ABL p190, Quantitative PCR

Clinical System Name

BCR/ABL p190, Quant

Synonyms

Philadelphia chromosome

t(9;22)

translocation (9;22)

Description

This test is for monitoring patients who are being treated for chronic myeloid leukemia (CML) or Philadelphia chromosome positive.

Sample Requirements

Specimen:  Whole Blood or Bone Marrow

Container(s):  Lavender/EDTA (Dk Green/Sodium Heparin NOT acceptable)

Preferred Vol: 10 mL blood; 2 mL bone marrow

Minimum Vol:  6 mL blood; 1 mL bone marrow; no micro collection

 

Note:  Record specimen type and date/time of collection on label.

For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing .

Processing Instructions

Reject due to:  wrong anticoagulant

Spin:  NO

Aliquot:  NO

Temp:  refrigerate

Notes:  Affix large label to vacutainer.

Storage location:  Store sample in CPA refrigerator sendouts rack.  On weekends send sample refrigerated to UW NW220 ASAP so that sample can be run first thing Monday morning.

 

Off-site collection:  Contact UW Hematopathology at (206) 288-7060 for instructions.

Stability

Specimen Type Temperature Time
  Room temp <1 day
  Refrigerated specimens received at UW after ~12noon Friday will be refrigerated over weekend for set up Monday (not preferred)
  Frozen NO

 

Availability

STAT Performed TAT
N Drawn daily.  Performed Wednesday, results Thursday. 2 weeks from day after receipt

 

Performing Laboratory

UW Molecular Hematopathology

Department

Department:  UW Hematopathology Laboratory, 825 Eastlake Ave E, Seattle, WA, 98195

Phone Number:  (206) 288-7060

 

Reference Range

none specified

Methodology

Method:  LDT, RT-PCR

Analytical Volume:  None specified

Limitations:  None specified

CPT Codes

CPT

Special Instructions

Links to:

Consent Forms

Algorithms

Requisition

Place test orders in CIS.

For bone marrow, use CIS order "HemOnc Bone Marrow Requisition" or "Hematopoietic Transplant Marrow Requisition", as appropriate.

Clinical Utility

x

Send Out Instructions

 

Reference Test Name: BCR/ABL p190, Quant by RT PCR
Reference Test Number: QP190
Instructions: Send out Monday through Friday with the UW courier.  Keep a copy of the Batch Sheet for our records.