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Test Code BCR/ABL p210 BCR/ABL p210, Quantitative PCR

Clinical System Name

BCR/ABL p210, Quant

Synonyms

BCR-abl detection at FHCRC

Philadelphia chromosome

t(9;22)

translocation (9;22)

Description

This test is for monitoring patients who are being treated for chronic myeloid leukemia (CML) and are known to have the p210 (b3:a2 or b2:a2) fusion form. 

Sample Requirements

Specimen:  Whole Blood or Bone Marrow

Container(s):  Lavender/EDTA (Dk Green/Sodium Heparin NOT acceptable)

Preferred Vol:  6 mL blood; 1-2 mL bone marrow

Minimum Vol:  5 mL blood; no micro collection

 

Note:  Record specimen type and date/time of collection on label.

For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing .

Processing Instructions

Reject due to:  wrong anticoagulant

Spin:  NO

Aliquot:  NO

Temp:  refrigerate

Notes:  Affix large label to vacutainer.

Storage location:  Store sample in CPA refrigerator sendouts rack.  On weekends send sample to UW NW220 refrigerated ASAP so that sample can be run first thing Monday morning.

 

Off-site collection:  Contact UW Hematopathology at (206) 288-7060 for instructions.

Stability

Specimen Type Temperature Time
  Room temp <1 day
  Refrigerated specimens received at UW after ~12noon Friday will be refrigerated over weekend for set up Monday (not preferred)
  Frozen NO

 

Availability

STAT Performed TAT
N Drawn daily. 2 weeks from day after receipt

 

Performing Laboratory

UW Hematopathology

Department

Department:  UW Hematopathology Laboratory, 825 Eastlake Ave E, Seattle, WA, 98195

Phone Number:  (206) 288-7060

 

Reference Range

none specified

Methodology

Method:  Cepheid GeneXpert Quantitative (Real-Time) Reverse Transcriptase PCR

Analytical Volume:  None specified

Limitations:  None specified

CPT Codes

CPT

Special Instructions

Links to:

Consent Forms

Algorithms

Requisition

Place test orders in CIS.

For bone marrow, use CIS order "HemOnc Bone Marrow Requisition" or "Hematopoietic Transplant Marrow Requisition", as appropriate.

Clinical Utility

Quantitative p210 BCR/ABL1 testing is performed using the Cepheid GeneXpert system which is able to detect 1 BCR/ABL1 positive cell in 100,000 normal cells. The result is reported as a percent ratio of BCR/ABL1 RNA to ABL1 RNA.

Send Out Instructions

 

Reference Test Name: BCR/ABL p210, Quantitative
Reference Test Number: CPHDQ
Instructions: Send out Monday through Friday with the UW courier.  Keep a copy of the Batch Sheet for our records.