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Test Code BKV PCR Quant BK Virus PCR Quant, Blood

Biohazard Infectious

Clinical System Name

BK Virus PCR Quant


BK Virus PCR Quant


BK Virus DNA is detected via nucleic acid amplification. Quantitative nucleic acid detection assays offer analytic sensitivity to detect infection at an early stage before clinically significant disease occurs.  The quantitative nature of these assays also allow threshold levels that can be used to monitor or begin treatment.


This test is validated for blood plasma only, serum will be accepted but may not be optimal for this assay. The sample may be shared with other Viral PCR tests. For Bone Marrow or other tissue, see BK Virus Qualitative PCR Assay. Please forward requests for BK Virus PCR Quant to the Microbiology Laboratory.

Sample Requirements

Specimen: Whole Blood

Container: Lavender/EDTA

Preferred Vol: 3.0 mL

Minimum Vol: 1.0 mL

Note: When drawing for multiple PCR tests (ex. CMV PCR, EBV PCR, BKV PCR), EACH PCR test requires 1.0 mL EDTA whole blood.


Heparin whole blood (Green Top Tubes) are not acceptable and will be rejected.

Processing Instructions

CPA: Send whole blood to Microbiology. The sample may be shared with other Viral PCR tests but cannot be shared or added on to samples that have been run through any analyzer. For shared whole blood samples, CPA will aliquot for all testing prior to sending samples to Microbiology. 


Off-site collection:  Off-site Children's Lab locations should spin down EDTA whole blood and aliquot the plasma for storage and transport to the SCH Microbiology Laboratory. Sample should be frozen at -20C for both storage and transport.                                        


Other off-site locations, it is recommended that the whole blood sample be spun down to obtain EDTA plasma. The EDTA plasma is stable for up to one week when stored at -20C and is the preferred sample for testing from off-site locations. 


Specimen Temp Time
Whole blood Room temp 2 h
  Refrigerated 24 h
  Frozen N
Plasma Refrigerated 24 h
  Frozen 1 week




Procedure Performed Turn Around Time (TAT)

24 - 96 hours


Testing is performed Tues, and Fri at 5 am. Test is not available STAT.

Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes



Reporting Information

Final Report: 24 -96 hours


Reference Ranges

Result Interpretation
None Detected Negative
1 - 499 Copies/mL See Comment*
≥500 Copies/mL Positive


*BKV: The limit of quantitative detection (the minimum virus level that gives a positive result in 95% of replicates) is 500 copies/mL (or 2.7 log cp/mL). Quantitative results less than 500 copies/mL (or 2.7 log cp/mL) are described as very low positive. The clinical significance of very low positive results is uncertain and should be individually analyzed with clinical discretion.