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Test Code BRAF UM BRAF Mutations

Important Note

This test detects the V600E and V600K mutations in the BRAF gene.

Clinical System Name

BRAF Mutations

Synonyms

V600E mutation, V600K mutation

Sample Requirements

EITHER (a) slides OR (b) tissue block:


(a) Instructions for slide specimens:
1 slide at 4-micron thickness stained with hematoxylin-and-eosin AND
10 unstained, non-baked slides at 10-micron thickness (a minimum of 5 unstained slides is acceptable).
Unstained slides can be on charged or uncharged slides.
Note: sections should contain as much tumor as possible.

(b) Instructions for tissue block specimen:
Provide complete tissue block containing tumor tissue.
If there is more than one tissue block, please provide the block that has the greatest amount of tumor tissue.  Tissue block will be returned at completion of testing.

 

(c) Add-on after prior testing in lab: contact UW Genetics lab

Processing Instructions

Reject due to: Decalcified sample

Spin: N

Aliquot: N

Temp: 2-8 C

Storage location: Affix large Cerner labels to tubes and place in refrigerator send-outs rack.

 

Off-site collection: Send whole blood refrigerated. Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood

Stability

Specimen Type Temperature Time
Whole blood Room temp 48 h
Whole blood Refrigerated 7 d
Whole blood Frozen N
Extracted DNA Room temp 3-4 d
Extracted DNA Refrigerated 1 y
Extracted DNA Frozen Indefinitely

 

Availability

 

STAT Performed TAT
N Accepted Mon - Fri from 0800 - 1500.  Performed once a week. 2-3 w

 

Performing Laboratory

University of Washington

Dept of Laboratory Medicine

UW Genetics Lab

1959 NE Pacific St, NW220
Seattle, WA 98195
 

Phone Number: (206) 520-4600

Department

Department: Send Outs

 

Phone Number: (206) 987-2563

Reference Range

Interpretive report is provided.

Methodology

Method: Exon 15 of the BRAF gene is analyzed by melting curve analysis to detect the V600E (GTG to GAG) and V600K (GTG to AAG) mutations in a modified protocol based on Ikenoue Cancer Genetics and Cytogenetics 2004;149:68–71. The test can normally detect a heterozygous mutation if it is present in more than about 5% of the cells in the sample. This test was developed and its performance characteristics determined by the Department of Laboratory Medicine at the University of Washington.

Analytical Volume: None specified

Limitations: None specified

CPT Codes

81210           (updated 1/16/17 by sstasi) 

Clinical Utility

This test detects mutations in exon 15 of the BRAF gene, which includes codon 600. Somatic mutations in BRAF occur in about 50% of melanomas and about 10-15% of colon cancers and are associated with responsiveness to BRAF inhibitors, and resistance to antibody therapy against the EGF receptor. This test can normally detect a heterozygous mutation if it is present in more than about 5% of the cells in the sample.

 

Send Out Instructions

Reference Test Name: BRAF Mutations
Reference Test Number: BRAF
Instructions: Send out M - F with the UW courier.