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Test Code Beta Hg Seq Beta Hemoglobin Sequencing

Clinical System Name

Beta Hemoglobin Sequencing

Synonyms

Beta Hb
Hb Beta
HBSEQ

Beta Globin

HBB

Sample Requirements

Whole blood is preferred

 

Specimen: Whole Blood

Container(s): Lavender/EDTA or Yellow/ACD

Preferred Vol: 5.0 mL

Minimum Vol: 2.0 mL

 

Specimen: Extracted DNA

Container(s): Sterile Plastic Tube

Preferred Vol: 30 ul at a concentration of 100ng/ul concentration

Minimum Vol: 20 ul at a concentration of 100ng/ul

 

Note:  For other specimen types contact the UW Genetics Lab (685-6429).

Processing Instructions

Reject due to: Dark Green/Sodium Heparin or Formalin-fixed paraffin-embedded tissues

Spin: N

Aliquot:N

Temp: 2-4 C

Storage location: Do not spin or separate specimen. Store whole blood affixed with a large computer label in the CPA refrigerator 1 send-outs rack.

 

Off-site collection: Do not spin or separate specimen. Transport refrigerated.

Stability

Specimen Type Temperature Time
Whole blood Room temp 3 d
Whole blood Refrigerated 7 d
Whole blood Frozen N
Extracted DNA Room temp 3-4 d
Extracted DNA Refrigerated 1 y
Extracted DNA Frozen Indefinitely

 

Availability

STAT Performed TAT
N Collected daily, performed weekly at UW 3 w

 

Performing Laboratory

University of Washington

Dept of Laboratory Medicine

Genetics Lab

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600
 

Department

Department:  Send Outs
 

Phone Number: (206) 987-2563

 

Reference Range

Interpretive report provided.
 

 

 

 

Methodology

Method: Sequences the coding regions and introns of the beta-globin gene (HBB) in both directions, identifies hemoglobin variants that are not easily diagnosed by electrophoresis/HPLC and can determine the cause of beta-thalassemia.

Analytical Volume: 1.0 mL

Limitations:

Send Out Instructions

 

Reference Test Name: Beta Hemoglobin DNA Sequencing
Reference Test Number: HBSEQ
Instructions: Send out M - F with the UW courier.