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Test Code Bld Malign Peripheral Blood Malignancy Workup by Flow Cytometry

Important Note

Specimens for PB Malignancy may be collected any time, to be tested the same day or held for testing the following working day. For specimens collected on weekends, holidays, or evening before a holiday, the ordering provider will be responsible for contacting the pathologist on call to arrange workup outside of regular lab hours; otherwise testing will be performed on the next regular working day.

For further information, contact Hematopathology (Cell Markers) at 206-987-2560 or Pathology 987-2103.

Clinical System Name

Blood Flow Malignancy Workup Request

Synonyms

Blood Malignancy Flow Workup Request
Flow Cytometry Immunophenotyping (Leukemia)
Immunophenotyping
Malignancy, Peripheral Blood
Peripheral Blood Malignancy Workup by Flow Cytometry
Routine Immunophenotyping

Description

Peripheral Blood Malignancy Workup (by Flow Cytometry) is used in determining the lineage of hematopoietic precursors and in classifying acute leukemias. This workup may be performed on peripheral blood if there are sufficient blasts for evaluation; requests will be evaluated by pathologist.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; Dark Green/Heparin also acceptable

Preferred Vol:  5 mL; volume dependent on blast count 

Minimum Vol:  dependent on blast count; consult lab

Note:  Specimens for PB Malignancy may be collected any time, to be tested the same day or held for testing the following working day.  This test is for peripheral blood workups only - for bone marrow, see separate listing.  peripop        

 

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location:  Days: M - F until 1630, transport specimen and labels to 10th floor Cell Markers (station #280); call before tubing specimen to make sure there is someone available to do testing.

After hours: Store sample in Cell Markers RT box in CPA. 

 

Off-site collection:  Keep specimen at room temperature and transport to Children's Lab.  Please contact Laboratory Client Services at (206) 987-2617 with method of transport and shipping number.

Stability

Temperature Time
Room temp 30 hrs in EDTA or 48 hrs in heparin
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
Y - with pathologist approval

Monday - Friday 0800-1630; Other times as arranged through pathologist on-call.  Weekend, holiday, pm before holiday, provider must contact pathologist on-call. SEE ORDERING NOTES ABOVE.

2-3 days

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

 

Reference Range

None specified
 

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on blast count; consult lab

Limitations:  None specified

CPT Codes

contact Laboratory Client Services at (206)-987-2617