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Test Code C Fungal Fungal Culture

Biohazard Infectious

Clinical System Name

Fungal Culture

Synonyms

Yeast

Filamentous Fungus

Thrush

Candida

Description

Includes Fungal culture and direct smear (either KOH w/calcofluor or Gram Stain depending on adequacy of specimen).  If sample is for suspected thrush or rule out yeast only, see Yeast Screen.

 

For hair, skin, or nail samples for Dermatophyte culture see C FUNGAL DERM

For blood samples where filamentous fungus is suspected (Aspergillis, Coccidiodes, Fusarium, Histoplasma, and Blastomyces) see Blood Culture, Fungal.

For blood samples where yeast are suspected see Blood Culture, Aerobic + Yeast.

 

Sample Requirements

SCH Main Campus Specimen Collection: Fluids, aspirates, and tissue samples should be collected and transported in a sterile screw-capped container (or syringe). Specimens received on swabs are sometimes inferior to samples of tissue or fluid, but will be accepted if collected using BD ESwab collection system. Place the swab into the Amies transport media tube provided in the collection kit.

 

Off-Site Specimen collection:  Fluids, aspirates, and tissue samples should be collected and transported in a sterile screw-capped container (or syringe). Specimens received on swabs are sometimes inferior to samples of tissue or fluid, but will be accepted if collected using BD ESwab collection system. Place the swab into the Amies transport media tube provided in the collection kit.

 

 

Preferred Vol: N/A

Minimum Vol: N/A

 

Processing Instructions

CPA: Send sample to Microbiology for processing. If sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology.

 

Off-site processing:  Fungal cultures are not set up at Off-site Children's Lab locations. Fluids, aspirates, and tissue samples that have been collected and transported in a sterile container or syringe should be sent via Delivery Express to Main Campus to allow set up within approximately 1 hour. Overlay any large tissue samples with Thio Broth and transport at room temp. 

 

Smaller pieces of tissue can be placed into ESwab Amies transport media. Small tissue samples, or other samples collected on ESwabs can be held at room temperature before and during transport. Samples should be received into the SCH Lab within 24 hours of collection.

The Regional Labs do not stock media for Fungal culture set up, so if there is an anticipated delay in specimen transport best to send via Delivery Express to Main Campus.

 

 

Stability

Tissue, Fluid or Syringe

Temp Time
Room temp

1 hour

Refrigerated N
Frozen N

 

 

BD ESwab

Temp Time
Room temp 24 hours  
Refrigerated OK up to 48 hours
Frozen N

 

 

Availability

Procedure Performed Turn Around Time (TAT)
KOH/ Gram Stain 24/7

24 hours

Culture 24/7 4 weeks

Cultures are read at appropriate intervals to detect early growth. STAT requests are not applicable.

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87102

 

This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)

Reporting Information

Negative Preliminary Report: 24 - 48 hours

Negative Final Report: 4 weeks

 

The report will be updated and the provider will be notified if growth is detected within the 4 week incubation period. 

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf