Sign in →

Test Code C PLEURAL Pleural Fluid Culture

Biohazard Infectious

Clinical System Name

Pleural Fluid Culture


Includes Bacterial Culture and Gram Stain. Specimen cultured for Aerobic and Anaerobic bacteria.

Sample Requirements

Specimen: Pleural Fluid

Container: Sterile Container

Preferred Vol: 1 mL

Minimum Vol: 0.2 mL

Processing Instructions


CPA: Send sample to Microbiology for processing. If the sample is shared and only one tube, syringe or other container is received, affix shared sample label and send entire sample plus requisition to Microbiology .


Off-site collection:  Off-site Children's Lab locations should set up culture and perform gram stain. Culture must be set up before remaining sample is refrigerated for courier pickup. If additional testing is requested such as AFB or Fungal culture, sample should be refrigerated for courier transport to SCH Micro for set up.


Other off-site locations, keep sample at room temperature until delivered and set up.


Conditions Time
Room temp 1 hour
Refrigerated N




Procedure Performed TAT
Gram Stain 24/7

1 hour

Culture 24/7 48 - 72 hours


Gram Stains on sterile fluids are performed STAT with a TAT of 1 hour.

Cultures are read at appropriate intervals to detect early bacterial growth. STAT requests are not applicable.

Performing Laboratory

Seattle Children's Laboratory



Microbiology Laboratory

CPT Codes




This test is not limited to the CPT codes listed.  Others may be added as more testing is necessary and depending on the pathogens isolated (such as MIC's and ID's)

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:


See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

Reporting Information

Any growth on a sterile fluid is considered positive and reported.


Preliminary Report:  18 - 24 hours

Negative Final Report: 48 hours


When Moderate to Abundant Polys are seen on Gram Stain, culture incubation will be extended up to one week.