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Test Code C3 C3 Complement

Clinical System Name

C3 Complement

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Gold Microtainer, Red, Lt. Green/Lithium Heparin, Dark Green/Sodium Heparin, Lavender/EDTA

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note: Avoid hemolysis.  C3 and C4 can share volume.

Processing Instructions

Reject due to

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: CPA refrigerator, FrigA rack.


Off-site collection: Spin and aliquot serum/plasma in plastic tube. Send refrigerated.


Specimen Type Temperature Time
gel separator with serum/plasma Refrigerated 48 h
separated serum/plasma Room temp 2 h
  Refrigerated 8 d
  Frozen -20 C 3 m



STAT Performed TAT
N M, W, F, day shift 1-3 d


Performing Laboratory

Seattle Children's Laboratory    


Department:  Chemistry

Location: Chem East

Phone Number:  (206) 987-2617

Reference Range


Newborn - 1 Month 55 - 129
2 - 3 Months 61 - 155
4 - 12 Months 64 - 187
> 1 Year 83 - 203



Method: Nephelometry by Siemens ProSpec

Analytical Volume: 0.250 mL serum/plasma

Limitations: Test is affected by repeated freeze-thaw cycles.  

CPT Codes