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Test Code CBC DIFF CBC + DIFF

Clinical System Name

Complete Blood Count with Differential

Description

Examination of the numerical complete blood count is useful in diagnosis of such disease states as anemias, leukemias, allergic reactions, viral, bacterial, and parasitic infections.

 

This test includes WBC, RBC, Hgb, Hct, MCV, MCH. MCHC, RDW, Plt, MPV, red cell morphology, white cell differential and Absolute Neutrophil Count (ANC).

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  1.0-3.0 mL

Minimum Vol:  0.5 mL microtainer; 1.0 mL macrotainer

 

Notes: A Lavender top/EDTA vacutainer must contain at least 1.0 mL.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample

Spin: N

Aliquot: N

Temp: RT

Storage location:  Deliver to Core Lab Hematology bench.

 

Off-site collection: Store whole blood at room teperature and transport to Children's Lab immediately.  Samples > 24 hours old may be rejected.

Stability

 

Specimen Type Temperature Time
 Whole blood RT

24 hrs

 Whole blood 2-8oC

 48 hrs

Whole blood -20oC or -70oC

N/A

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Hematology

Phone Number: 206-987-2561

 

 

Reference Range

Please refer to "CBC No Diff"  for Red cell indicies reference ranges.

 

Test

Age Range (M & F)

%

/CMM

Critical Values

Absolute Neutrophil Count

 

 

 

<500/CMM

 

 

 

 

 

Polys

0 - 1 Day

33 - 70

3000 - 12000

 

Polys

1 Day - 1 Week

15 - 50

2000 - 6000

 

Polys

1 Week - 1 Month

15 - 45

1500 - 5000

 

Polys

1 Month - 1 Year

15 - 45

1500 - 5000

 

Polys

1 - 4 Years

15 - 70

1500 - 7500

 

Polys

4 - 10 Years

25 - 70

1800 - 7000

 

Polys

10 - 14 Years

30 - 70

1500 - 7000

 

Polys

14 Years - Adult

40 - 75

1500 - 7000

 

Bands

0 - 24 Hrs

0 - 9

0 - 1500

 

Bands

1 Day - 1 Week

0 - 9

0 - 1200

 

Bands

1 Week - 1 Month

0 - 9

0 - 500

 

Bands

1 Month - 1 Year

0 - 9

0 - 350

 

Bands

1 - 4 Years

0 - 9

0 - 270

 

Bands

4 - 10 Years

0 - 9

0 - 240

 

Bands

10 Years - Adult

0 - 9

0 - 200

 

Lymphocytes

0 - 1 Day

10 - 35

2000 - 11000

 

Lymphocytes

1 Day - 1 Week

15 - 70

2000 - 7000

 

Lymphocytes

1 Week - 4 Years

30 - 70

3000 - 7000

 

Lymphocytes

4 - 6 Years

20 - 70

1500 - 8500

 

Lymphocytes

6 - 10 Years

20 - 50

1500 - 5000

 

Lymphocytes

10 Years - Adult

20 - 40

1100 - 4500

 

Monocytes

0 Minutes - 1 Month

0 - 10

0 - 1100

 

Monocytes

1 Month - 150 Years

0 - 10

0 - 900

 

Eosinophils

0 - 24 Hrs

0 - 4

0 - 400

 

Eosinophils

1 Day - 1 Week

0 - 4

0 - 500

 

Eosinophils

1 Week - 1 Year

0 - 4

0 - 300

 

Eosinophils

1 - 10 Years

0 - 4

0 - 250

 

Eosinophils

10 Years - Adult

0 - 4

0 - 200

 

Basophils

0 - 1 Week

0 - 1

0 - 100

 

Basophils

1 Week - 4 Years

0 - 1

0 - 50

 

Basophils

4 Years - Adult

0 - 1

0 - 40

 

Immature Granulocytes   0 - 0.7 0 - 50  

 

 

Methodology

Method:  Sysmex XN-3000 & XN-1000: RF/DC detection method, Flow cytometry with semiconductive laser, photometrically, SLS-hemoglobing method, hydrodynamic focusing

Analytical Volume:  0.25 mL  whole blood

 

CPT Codes

85025

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