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Test Code CD40 Prot Exp CD40 Protein Flow

Important Note

This test is no longer offered as a clinical test. It is Research Use Only (RUO). For more information contact the Immunology Diagnostics Laboratory (IDL) at idl@seattlechildrens.org.

Clinical System Name

CD40 Protein Flow

Synonyms

Hyper IgM Syndrome, HIGM

CD40, CD40 molecule, p50, Bp50, CDW40W, TNFRSF5

Description

CD40 is a member of the TNF-receptor superfamily.  It is important in mediating immune and inflammatory responses; this includes T cell-dependent immunoglobulin class switching, memory B cell development and germinal center formation.  The interaction of CD40 and it's ligand is important for amyloid-beta-induced microglial activation.  This assay determines if the patient B cells express the CD40 protein compared to normal B cells.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Dark Green/Sodium Heparin (no serum separator)

Preferred Vol:  10-20 mL

Minimum Vol:  5-7 mL (for young children only)

 

Note: Specimens stable for only 24 hours at room temperature.

 

 

 

Collected in-house at

Seattle Children's

Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday must arrive in main lab before 9 AM to reach IDL before 12 noon must be received at IDL before 12 noon Friday
Saturday do not collect do not collect
day before a holiday must arrive in main lab before 9 AM to reach IDL before 12 noon must be received at IDL before 12 noon day before holiday
Sunday

ok if collected after 12 noon - must be received at IDL before 12 noon next day

ok if collected after 12 noon - must be received at IDL before 12 noon next day

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon - must be received at IDL before 12 noon next day

ok if collected after 12 noon - must be received at IDL before 12 noon next day

 

 

Processing Instructions

Reject due to: Clotted specimens, microtainers, refrigerated, samples received > 24 hours post-collection

Spin: N

Aliquot: N

Temp: Room Temperature

Storage location: Notify Sendouts Lab immediately.  Place whole blood in CPA room temp send-outs rack. Store extra labels in the clear plastic box attached to CPA refrigerator.

 

Off-site collection: It is critical that the samples be kept at room temperature; use extra packing to maintain temperature.  If specimen will not arrive at Immunology Diagnostic Laboratory before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning.  Testing must be set up within 24 hours of collection.  The lab accepts samples Monday through Friday but samples should be shipped only Monday through Thursday so they do not sit over the weekend. 

 

Transport sample at room temperature via overnight shipping to:

 

 Seattle Children's Research Institute

Immunology Diagnostic Laboratory

1900 9th Avenue JMB-7

Seattle, WA 98101-1304 

Stability

Specimen Type Temperature Time
Whole Blood Room temp 24 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N Monday - Thursday; must be in lab before noon 4-6 weeks

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department:  Immunology Diagnostic Laboratory

Address:  1900 9th Avenue JMB-7, Seattle, WA 98101-1304

Phone: 206-987-7435

Email: idl@seattlechildrens.org

 

Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org

Reference Range

Reference values accompany patient report.

Methodology

Isolated PBMCs are cell-surface stained with fluorescently-labeled antibodies to CD19 and CD40 then evaluated by flow cytometry.

 

Method: Flow Cytometry

Analytical Volume: 5 mL

CPT Codes

88184, 88185 (x2), 88187

Special Instructions

Links to:  Immunology Diagnostic Laboratory

Requisition

IDL Requisition