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Test Code CD40L Prot Exp CD40 Ligand Protein Flow

Important Note

This test is no longer offered as a clinical test. It is Research Use Only (RUO). For more information contact the Immunology Diagnostics Laboratory (IDL) at idl@seattlechildrens.org.

Clinical System Name

CD40 Ligand Protein Expression

Synonyms

Hyper IgM Syndrome, HIGM

RP3-527F8.3, CD 154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L

Description

CD40 Ligand is expressed on the surface of activated T cells.  It binds to CD40 on the surface of B cells to initiate immunoglobulin class switching.  Mutations in CD40L cause X-Linked Hyper IgM syndrome Type 1.  This panel test for the expression of CD40L on activated T cells with 2 antibodies from different clones that bind at different epitopes of the CD40L molecule.  It also tests the functional ability of CD40L to bind to CD40 on the surface of B cells to initiate immunoglobulin class switching.   

Sample Requirements

Specimen:  Whole Blood

Container(s):  Dark Green/Sodium Heparin (no serum separator)

Preferred Vol:  10-20 mL

Minimum Vol:  5-7 mL (for young children only)

 

Note: Specimens stable for only 24 hours at room temperature.

 

 

 

Collected in-house at

Seattle Children's

Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday must arrive in main lab before 9 AM to reach IDL before 12 noon must be received at IDL before 12 noon Friday
Saturday do not collect do not collect
day before a holiday must arrive in main lab before 9 AM to reach IDL before 12 noon must be received at IDL before 12 noon day before holiday
Sunday

ok if collected after 12 noon - must be received at IDL before 12 noon next day

ok if collected after 12 noon - must be received at IDL before 12 noon next day

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon - must be received at IDL before 12 noon next day

ok if collected after 12 noon - must be received at IDL before 12 noon next day

 

 

Processing Instructions

Reject due to: Clotted specimens, microtainers, refrigerated, samples received > 24 hours post-collection

Spin: N

Aliquot: N

Temp: Room Temperature

Storage location: Notify Sendouts Lab immediately.  Place whole blood in CPA room temp send-outs rack. Store extra labels in the clear plastic box attached to CPA refrigerator.

 

Off-site collection: It is critical that the samples be kept at room temperature; use extra packing to maintain temperature.  If specimen will not arrive at Immunology Diagnostic Laboratory before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight to allow test set-up the following morning.  Testing must be set up within 24 hours of collection.  The lab accepts samples Monday through Friday but samples should be shipped only Monday through Thursday so they do not sit over the weekend. 

 

Transport sample at room temperature via overnight shipping to:

 

 Seattle Children's Research Institute

Immunology Diagnostic Laboratory

1900 9th Avenue JMB-7

Seattle, WA 98101-1304 

Stability

Specimen Type Temperature Time
Whole Blood Room temp 24 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N Monday - Thursday; must be in lab before noon 4 -6 weeks

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department:  Immunology Diagnostic Laboratory

Address:  1900 9th Avenue JMB-7, Seattle, WA 98101-1304

Phone: 206-987-7435

Email: idl@seattlechildrens.org

 

Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org

Reference Range

Reference values accompany patient report.

Methodology

Establish expression of CD40L using two monoclonal antibodies and competence of binding with CD40 in patient CD4 T cells compared to normal CD4 T cells.

 

Method: Flow Cytometry

 

CPT Codes

86353 (x2), 88184, 88185 (x5), 88187

Special Instructions

Links to:  Immunology Diagnostic Laboratory

Requisition

IDL Requisition