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Test Code CF TOBRA Tobramycin - Cystic Fibrosis Patients

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

Clinical System Name

Cystic Fibrosis Tobramycin Level

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Red, Dark Green/Sodium Heparin, or Lavender/EDTA

Preferred Vol: 1 mL

Minimum Vol:  1 Lt. Green/Li Heparin Microtainer,1 Gold Microtainer, or 1 Lavender/EDTA Microtainer

 

Note: Every 24 hour dosing: Infuse IV over 30 minutes. Upon admission, initial levels should be drawn following the 2nd dose. Levels should be drawn at 2 and 6 hours after the end of the IV infusion. Document the date and the time of infusion and collections. Following a dose adjustment during a single admission, repeat 2 and 6 hour levels should follow the 1st dose of the new dose regimen.

Processing Instructions

Reject due to:  Insufficient quantity

Spin: Y

Aliquot: Y

Temp: 2-4oC

Storage location:  Core Chemistry Refrigerator

 

Off-site collection:  Every 24 hour dosing: Infuse IV over 30 minutes. Upon admission, initial levels should be drawn following the 2nd dose.  Levels should be drawn at 2 and 6 hours after the end of the IV infusion. Document the date and the time of infusion and collections. Following a dose adjustment during a single admission, repeat 2 and 6 hour levels should follow the 1st dose of the new dose regimen. Spin blood, freeze plasma or serum.

Stability

 

Specimen Type Temperature Time
Serum or Plasma RT

2 H

Serum or Plasma 2-4oC

7 D

Serum or Plasma -20o C or -70oC

14 D

 

Availability

STAT Performed TAT
Y 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2578

 

 

Reference Range

AUC*:  80 - 120 mg/hr/L

 

*Note: AUC is calculated by pharmacy (987-2033) and documented in inpatient progress note.


 

Methodology

Method:  Two Point Rate method by Vitros 4600

Minimum Volume: 0.1 mL serum or plasma

Analytical Volume: 0.06 mL serum or plasma + 0.03 mL dead space

Critical Values

0 - 1 year:   > 12 mcg/mL

Greater than 1 year:  > 30 mcg/mL

CPT Codes

80200