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Test Code CH-KARYFIB Chromosomes - Fibroblasts

Clinical System Name

Fibroblast Culture specimen

Synonyms

Cytogenetics
Fibroblast Karyotyping
Karyotype Fibroblast
Fibroblast Karyotyping

Fibroblasts

Fibroblast culture

Description

This test is usually performed when mosaicism is suspected.  Skin or tissue may be cultured in order to establish long term single cell cultures to detect abnormalities in chromosome number, large chromosomal duplications and deletions and other large structural rearrangements.  If mosaicism is suspected, biopsies from two sites is recommended.

Sample Requirements

Specimen: Skin or other tissue

Container(s): Cytogenetics transport media

Preferred Vol:  2.0 - 4.0 mm punch biopsy 

Minimum Vol:  2.0 mm punch

Note:  Obtain fibroblast transport medium from Lab (x72102).  Check tube for expiration date. Specify skin biopsy site.  DO NOT USE FORMALIN. Transport media is RPMI with fetal bovine serum and antibiotics.  It can be used for Cytogenetics and Cell Markers

Processing Instructions

Reject due to: n/a - send to lab

Spin: N

Aliquot: N

Temp: RT

Storage location:  Process sample without delay.  Days: Transport specimen, requistion, and labels to main 8th floor laboratory.  Eves/Nights: Store in Molecular Genetics box in CPA refrigerator #2 with labels & requisitions.  DO NOT FREEZE FIBROBLASTS.

 

Additional information:  CPA:  transport media is stored in Histology frig #2 on door.  Media is pink in 15 mL tubes with blue screw cap. Check expiration date.  Back-up supply in Cell Markers Lab.

 

Off-site collection:  Obtain fibroblast transport medium from Lab (206-987-2102). Specify skin or tissue biopsy site. Keep sample sterile. Refrigerate if sample transport is delayed. Test request form must be completed by the ordering provider. Patient history information is required. Samples received after 3 pm will be set up the following day.

Stability

Temperature Time
Room temp ok
Refrigerated ok
Frozen No

 

Availability

STAT Performed TAT
N Daily 6 weeks

 

Performing Laboratory

Seattle Children's Hospital

Department

Department: Cytogenetics LaboraDepartment: Cytogenetics Laboratory 

Phone number: 206-987-3961

 

Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org

 

Lab Genetic Counselor: LabGC@seattlechildrens.org

 

Phone number: 206-987-3961

 

Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org

 

Lab Genetic Counselor: LabGC@seattlechildrens.org

 

Methodology

Method: Routine G-band analysis

CPT Codes

88233, 88285, 88262, 88291

Special Instructions

Specify skin or tissue biopsy site. Test request form must be completed by the ordering provider. Patient history information is required. For additional information or consultation call Children's Cytogenetics Lab at (206) 987-3961. After hours call the Clinical Lab at (206) 987-2102 or the on-call pathologist at (206) 987-2131.

 

Clinical Utility

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