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Test Code CH-SKIN Fibroblast Culture and Storage

Clinical System Name

Fibroblast Culture specimen

Synonyms

Skin Fibroblast Culture

Description

Solid tissues may be cultured in order to establish long term single cell cultures and frozen for storage in anticipation of future enzyme testing, DNA extraction or to rule out constitutional chromosome abnormalities.  Tissues of choice include skin, products of conception (including fetal, placental, or membrane tissue) or any other tissues with the ability to grow in culture (unacceptable sample would include brain and muscle tissues).

Sample Requirements

Specimen: Tissue

Container(s): Fibroblast Transport Media, Hank's Balanced Salt Solution or sterile saline (cell marker/cytogenetics transport media)

Preferred Vol:  2.0 - 4.0 mm punch biopsy 

Minimum Vol:  2.0 mm punch

 

Note:  Obtain fibroblast transport medium from Lab (x72102).  Check tube for expiration date. Specify skin biopsy site. Keep sample sterile and at room temperature or refrigerated; do not freeze. Test request form must be completed by the ordering provider. Patient history information is required. Samples received after 3 pm will be set up the following day.  Samples that are fixed in formalin or formaldehyde will not be accepted.

Processing Instructions

Reject due to: n/a - send to lab

Spin: N

Aliquot: N

Temp: RT

Storage location:  Process sample without delay.  Days: Transport specimen, requistion, and labels to 10th floor (station #180). Eves/Nights: Store in Molecular Genetics box in CPA refrigerator #2 with labels & requisitions.  DO NOT FREEZE.

 

Additional information:  CPA:  transport media is stored in Histology frig #2 on door.  Media is pink in 15 mL tubes with blue screw cap. Check expiration date.  Back-up supply in Cell Markers Lab.

 

Off-site collection:  Obtain fibroblast transport medium from Lab (206-987-2102). Specify skin biopsy site. Keep sample sterile. Refrigerate if sample transport is delayed. Test request form must be completed by the ordering provider. Patient history information is required. Samples received after 3 pm will be set up the following day. Samples must be set-up ASAP, plan collection & shipping accordingly - do not ship on ice.

Stability

Temperature Time
Room temp Shipping ok
Refrigerated Overnight ok
Frozen No

 

Availability

STAT Performed TAT
N Daily 6 weeks

 

Performing Laboratory

Seattle Children's Hospital

Department

Department: Cytogenetics Laboratory 

Phone number: 206-987-3961

 

Lab Client Services: 206-987-2617, labclientservices@seattlechildrens.org

 

Lab Genetic Counselors: LabGC@seattlechildrens.org

 

 

Methodology

Method: Tissue culture

CPT Codes

88233, 88240

Clinical Utility

x