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Test Code CSF Pyruvate Pyruvate, CSF

Clinical System Name

CSF Pyruvate

Description

CSF lactate and pyruvate are measured to calculate a lactate-to-pyruvate ratio in the investigation of possible mitochondrial disease.

Sample Requirements

Specimen: Cerebrospinal fluid

Container(s): Sterile Specimen Container

Preferred Vol: 1 mL

Minimum Vol: 1 mL

 

Note: Place the sample on ice and deliver to the lab within 10 minutes of collection.  Note if patient was crying, or if collection was difficult.

 

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: - 20 C

Storage location: Deliver to CORE within 2 min of receipt. A protein-free filtrate must be made by CORE as soon as possible.  

 

CORE: Add 0.5 mL CSF to 1.0 mL cold perchloric acid and mix by vortexing. Centrifuge and transfer supernate to plastic tube. Store in CPA Freezer, Fz5 rack.

 

Off-site collection:  Prepare protein-free filtrate: Add 0.5 mL CSF to 1.0 mL cold perchloric acid and mix by vortexing. Centrifuge and transfer supernate to plastic tube.  Send frozen.

Stability

Specimen Type Temperature Time
protein-free filtrate, CSF Room temp 1 w
  Refrigerated 1 w
  Frozen 1 m

 

Availability

STAT Performed TAT
N M, Th 1-5 d

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Chemistry

Location: Chem East

Phone Number: (206) 987-2617

 

Reference Range

All ages: 0.06 - 0.19 mmol/L

Methodology

Method: Enzymatic Rate Reaction by Vitros 4600

Analytical Volume: 0.5 mL

Limitations:

CPT Codes

84210