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Test Code CT PCR Chlamydia trachomatis PCR

Biohazard Infectious

Synonyms

Chlamydia PCR
Chlamydia trachomatis amplified probe

CT NG

Clincal System Name

Chlamydia trachomatis PCR

Description

This includes nucleic acid amplification for Chlamydia trachomatis with amplified probe technique.

Sample Requirements

Specimen: Dirty Catch Urine, Endocervical Swab, Vaginal Swab

Container:

  • Sterile Container for Urines
  • CT/NG Swab Kit (a.k.a. Xpert CT/NG Vaginal/Endocervical Specimen Collection Kit) for Vaginal and Endocervical sites

Preferred Vol: 10mL for Urine

Minimum Vol: 7.0mL for Urine

 

Please consult the Photo Swab Guide to determine which swab to collect.

 

First-catch urine or dirty catch urine with no genital cleaning (NOT mid-stream clean catch) is required. If the patient has submitted a clean catch sample, a dirty catch sample can be submitted on the following day.

 

Clean Catch Urines or Urines received in preservative or transport media are unacceptable for testing and will be REJECTED.

 

Bloody swabs/urine samples will be sent to University of Washington for testing.  Send bloody urine to lab in sterile container.  Collect bloody Vaginal or Endocervical samples with Gen-Probe APTIMA collection kit.

 

Samples received in Aptima collection kits will be forwarded to the University of Washington for testing.

 

This test is validated for urine, endocervical, and vaginal specimens only.  Other specimen sites such as eye, throat, urethral and anal will be accepted but are not optimal for this assay.  The preferred collection kit is the Xpert CT/NG (Vaginal/Endocervical) swab kit.  UTM is acceptable but not optimal. 

Processing Instructions

CPA: Send sample to Microbiology for processing. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

 

Main Campus collection:  Please consult the Photo Swab Guide to determine which swab to collect. 

 

 

Regional Clinic collection:  Please consult the Photo Swab Guide to determine which swab to collect. Send to SCH Main Campus for testing. Refrigerate before and during courier transport. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

Stability

Urine
Temp Time
Room temp 24 hours
Refrigerated 1 week
Frozen N

 

Vaginal/Endocervical Swabs
Temp Time
Room temp 60 d
Refrigerated 60 d
Frozen N

 

Availability

Procedure Performed Turn Around Time (TAT)
In House Cephied PCR 24/7 3-5 hours
APTIMA PCR (for bloody samples sent out to UW) 24/7

3 - 5 d

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87491

Reporting Information

Final Report: 3 - 5 d

 

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf