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Test Code CarnFreTo Carnitine Free and Total, blood

Clinical System Name

Carnitine Free and Total, Blood

Description

Measurement of free, acylcarnitine and total carnitine by ESI-MS/MS using stable isotope internal standards

 

 

Sample Requirements

Specimen: Whole Blood

Container(s): Dark Green/Sodium Heparin, Lt. Green/ Lithium Heparin, Gold SST, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note: Serum is also an acceptable sample type.

Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: -20 C

Storage location: -20 C Biochemical Genetics Box

 

Off-site collection: Freeze and ship heparinized plasma or serum. 

Stability

Temperature Time
Room temp 5 days
Refrigerated 5 days
Frozen (-200C) (serum/plasma) 1 month

 

Availability

STAT Performed TAT
N T, Th 3-7 days

 

Contact the Biochemical Genetics Lab for requests outside of stated availability (206)987-2216.

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Biochemical Genetics

Phone Number: 206-987-2216

 

 

Methodology

Method: Tandem mass spectrometry

Analytical Volume: 0.2 mL

Limitations:

CPT Codes

82379

 

Requisition

Biochemical Genetics Requisition

 

On the requisition include clinical information needed for appropriate interpretation.  (Age, gender, drug therapy, and family history)

Clinical Utility

Carnitine is quantitated for the purpose of follow-up in patients with organic acidurias or fatty acid oxidation disorders who may become carnitine deficient, those on valproate or other drugs that bind to and deplete carnitine, those on hyperalimentation or dialysis, and those on supplemental carnitine.

Reference Ranges

mcmol/L < 1 wk 1 wk - 1 mo > 1 mo
Total Carnitine 17 - 41 20 - 59 29 - 84
Free Carnitine 10 - 33 16 - 57 18 - 65
Acylcarnitine 3 - 24 4 - 24 4 - 28
Acyl/Free ratio 0.2 - 1.4 0.2 - 0.5 0.1 - 0.8