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Test Code CelHLA Celiac Associated HLA-DQ Alpha 1 and DQ Beta 1 DNA Typing, Blood

Additional Codes

HLA Typing for Celiac disease

Reporting Name

Celiac Associated HLA-DQ Typing
Seattle Children's Hospital Note:

Aliases:

HLA DQ2

HLA DQ8

 

Useful For

Assessing risk of celiac disease


Specimen Required


Container/Tube: Yellow top (ACD solution B)

Specimen Volume: 6 mL

Collection Instructions: Do not transfer blood to other containers.


Specimen Type

Whole Blood ACD-B
Seattle Children's Hospital Note:

ACD Solution A is acceptable, not preferred.

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated (preferred)
  Ambient 

Day(s) and Time(s) Performed

Monday through Friday; 7:30 a.m.-5:00 p.m.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probe (SSO)

Method Description

LABType applies Luminex technology to the reverse sequence-specific oligonucleotide (SSO) DNA typing method. First, target DNA is PCR-amplified using a group-specific primer. The PCR product is biotinylated, which allows it to be detected using R-phycoerythrin-conjugated streptavidin. The PCR product is denatured and allowed to rehybridize to complementary DNA probes conjugated to fluorescently coded microspheres. A flow analyzer identifies the fluorescent intensity of phycoerythrin on each microsphere. The HLA Class II allele or allele groups of the sample is determined by the positive and negative bead ID's using a computer software program. The assignment of the HLA typing is based on the reaction pattern compared to patterns associated with published HLA gene sequences.(Package insert: One Lambda, LABType SSO Typing)

Reference Values

An interpretive report will be provided.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

81376 x 2-HLA Class II typing, low resolution (eg, antigen equivalents); one locus (eg, HLA-DRB1/3/4/5, -DQB1, -DQA1, -DPB1, or -DPA1), each

 

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CELI Celiac Associated HLA-DQ Typing In Process

 

Result ID Test Result Name Result LOINC Value
DQA DQ alpha 1 44728-4
DQB DQ beta 1 43291-4
CELIG Celiac gene pairs present? 48767-8
CELIC Interpretation 59465-5

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

Testing Algorithm

The following algorithms are available in Special Instructions:

-Celiac Disease Comprehensive Cascade

-Celiac Disease Diagnostic Testing Algorithm

-Celiac Disease Gluten-Free Cascade

-Celiac Disease Routine Treatment Monitoring Algorithm

-Celiac Disease Serology Cascade

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)