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Test Code D-Lactate D-Lactate, Plasma

Important Note

This test is NOT available for collection at Seattle Childrens.  It requires collection in a grey-top tube that is not stocked at Seattle Children's locations.  Urine d-lactate is the preferred test for d-lactate acidosis.

Reporting Name

D-Lactate, P

Useful For

An adjunct to urine D-lactate (preferred), in the diagnosis of D-lactate acidosis


Specimen Required


Collection Container/Tube: Grey top (potassium oxalate/sodium fluoride) (Supply T275)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Spin down and immediately freeze specimen.

Additional Information: For L-lactate (lactic acid), order LLA / Lactate, Plasma.


Seattle Children's Hospital Note:

Obtain Grey Top from Main Lab. Collect 2.0 mL whole blood in a Grey top.

Specimen Type

Plasma NaFl-KOx

Specimen Minimum Volume

0.55 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma NaFl-KOx Frozen (preferred) 365 days
  Ambient  7 days
  Refrigerated  7 days

Day(s) and Time(s) Performed

Monday, Thursday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Enzymatic

Method Description

D-lactate is oxidized to pyruvate in the presence of D-lactate dehydrogenase and nicotinamide adenine dinucleotide phosphate (NAD). The reaction proceeds because the pyruvate is continually removed as a pyruvate-hydrazone complex. The quantity of reduced NAD produced is directly proportional to the amount of D-lactate oxidized and is measured spectrophotometrically at 340 nm.(Oh MS, Phelps KR, Traube M, et al: D-Lactic acidosis in a man with the short-bowel syndrome. N Engl J Med 1979;301:249-252; Dahlquist NR, Perrault J, Callaway CW, Jones JD: D-Lactic acidosis and encephalopathy after jejunoileostomy: response to overfeeding and to fasting in humans. Mayo Clin Proc 1984;59:141-145)

Reference Values

0.0-0.25 mmol/L

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83605

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLAC D-Lactate, P 14045-9

 

Result ID Test Result Name Result LOINC Value
8878 D-Lactate, P 14045-9

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Testing Algorithm

DLAU / D-Lactate, Urine is the preferred specimen for D-lactate determinations.