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Test Code DIR LDL LDL Cholesterol (Beta-Quantification), Serum

Reporting Name

LDL Cholesterol (Beta-Quant), S

Useful For

Evaluation of cardiovascular risk

 

Assessment of low-density lipoprotein C (LDL-C) in patients with hypertriglyceridemia, type III hyperlipoproteinemia/dysbetalipoproteinemia, or when an accurate gold standard determination of LDL-C is required

 

Diagnosis of familial hypobetalipoproteinemia and abetalipoproteinemia


Specimen Required


Patient Preparation: Patient must not consume any alcohol for 24 hours before the specimen is drawn.

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 3 mL

Additional Information: Indicate patient's age and sex.


Seattle Children's Hospital Note:

Collect 6.0 mL whole blood in a Red top.

Specimen Type

Serum

Specimen Minimum Volume

<2 years: 1 mL; ≥2 years: 2 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 10 days
  Frozen 

Day(s) and Time(s) Performed

Monday through Thursday, Saturday; 3 p.m. 

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Ultracentrifugation/Selective Precipitation/Enzymatic Colorimetry (Beta-Quantification)

Method Description

Ultracentrifugation is performed to remove chylomicrons and very low-density lipoproteins present in the supernatant. Separation of low-density lipoprotein particles is achieved via selective precipitation from high-density lipoprotein. Cholesterol is quantitated by an enzymatic, colorimetric method.(Ellefson RD, LR, Hodgson PA, Weidman, WH: Cholesterol and triglycerides in serum lipoproteins of young persons in Rochester, Minnesota. Mayo Clinic Proc 1978;53:307-320; Warnick GR, Benderson J, Albers JJ: Dextran sulfate-Mg2+precipitation procedure for quantitation of high-density-lipoprotein cholesterol. Clin Chem 1982;28[6]:1379-1388)

Reference Values

The National Lipid Association and the National Cholesterol Education Program (NCEP) have set the following guidelines for LDL-C in adults (ages 18 years and up):

Desirable: <100 mg/dL

Above Desirable: 100-129 mg/dL

Borderline high: 130-159 mg/dL

High: 160-189 mg/dL

Very high: ≥190 mg/dL

 

The Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents has set the following guidelines for LDL-C in children and adolescents (ages 2-17 years):

Acceptable: <110 mg/dL

Borderline high: 110-129 mg/dL

High: ≥130 mg/dL

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83701-Lipoprotein, blood; high resolution fractionation and quantitation of lipoproteins including lipoprotein subclasses when performed (eg, electrophoresis, ultracentrifugation)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LDLD LDL Cholesterol (Beta-Quant), S 18261-8

 

Result ID Test Result Name Result LOINC Value
LDLC LDL Chol (Beta-Quantification), S 18261-8

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross reject

Other

NA

Forms

If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/cardiovascular-request-form.pdf).