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Test Code Desmgl 1+3 Desmoglein 1 (DSG1) and Desmoglein 3 (DSG3), IgG Antibodies, Serum

Reporting Name

Desmoglein 1 and 3, S

Useful For

Preferred screening test for patients suspected to have an autoimmune blistering disorder of the skin or mucous membranes (pemphigus)

 

As an aid in the diagnosis of pemphigus

 

Monitoring treatment response in patients with a confirmed diagnosis of pemphigus

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  14 days
  Ambient  36 hours

Reference Values

DESMOGLEIN 1

<14.0 U (negative)

14.0-20.0 U (indeterminate)

>20.0 U (positive)

 

DESMOGLEIN 3

<9.0 U (negative)

9.0-20.0 U (indeterminate)

>20.0 U (positive)

Day(s) and Time(s) Performed

Once per week, Monday through Friday, day of testing determined by laboratory

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83516 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DSG13 Desmoglein 1 and 3, S 43309-4

 

Result ID Test Result Name Result LOINC Value
83680 DSG 1 43311-0
24027 DSG 3 43312-8

Method Description

This enzyme-linked immunosorbent assay (ELISA) method detects and measures serum levels of antibodies of certain pemphigus diseases. Calibrators and patient sera are added to microwells coated with DSG1 and DSG3 antigens, allowing antibodies to react with the immobilized antigens. After washing to remove any unbound serum proteins, horseradish peroxidase conjugated IgG is added and incubated. Following another wash step, the peroxidase substrate is added and allowed to incubate for an additional period. Stop solution is then added to each well to cancel the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically and plotting the results.(Ishii K, Amagai M, Hall RP, et al: Characterization of autoantibodies in pemphigus using antigen specific ELISA's with baculovirus expresses recombinant desmogleins. J Immunol 1997 Aug 15;159[4]:2010-2017)

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild reject; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)