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Test Code E QF TB Employee Quantiferon-TB Test

Clinical System Name

Quantiferon-TB Gold

Sample Requirements

Specimen: Whole blood

Container(s): 3 Quantiferon TB Tubes: Nil, TB Antigen, and Mitogen

Preferred Vol: Fill to black mark on each tube 

Minimum Vol: N/A

 

Note: Obtain special Quantiferon tubes and specific instructions from Laboratory (987-2102).  If butterfly needle is used, a "purge" tube should be collected to ensure tubing is filled before filling Quantiferon TB Gold tubes. After collection, shake tubes 10 times to coat the inner surface and deliver to the laboratory at RT.

 

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: RT

Storage location:

Time

Day

Action

00:00 – 8:00

Daily

Store in Biochemical Genetics RT box

08:00 – 16:00

M – F

Tube to OC.10

Sa – Su,

Holidays

Invert tubes 10 times and store upright in rack in CPA incubator.

Note incubation time start time on label.

16:00 – 23:59

Daily

Invert tubes 10 times and store upright in rack in CPA incubator.

Note incubation time start time on label.

 

Off-site collection:  Specimens must be collected in three Quantiferon TB Gold tubes and received within 16 h of collection.  Ship at RT.

Stability

Specimen Type Temperature Time
  Room temp 16 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N T, F 1 w

Call the Chemistry Laboratory (206-987-2565) for requests outside of stated availability. Because of the incubation time necessary for testing, results cannot be obtained prior to 36-48 hr post blood draw.

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Chemistry

Location: Chem West

Phone Number: (206) 987-2617

 

 

 

Reference Range

Expected value: Negative

Individuals on steroids may have indeterminate results. For indeterminate-low mitogen results a redraw is recommended.

Methodology

Method: Interferon-gamma release assay

Analytical Volume:

Limitations: