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Test Code ENA PANEL Extractable Nuclear Antigen Group (ENA)

Additional Codes

Clinical System Name

Extractable Nuclear Antigen Group

Synonyms

Anti ENA Panel
Anti Sm
Anti Sm RNP
Anti SS-A
Anti SS-B
ENA Antibodies
SSA
SSB

Description

This assay is used in the detection and quantitation of autoantibodies to aid in the diagnosis of autoimmune disease.  The ENA panel is intended for the detection of IgG class antibody to 5 separate analytes including SSA, SSB, Sm, RNP, and Sm/RNP in human sera.  The results are semi-quantitative.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Gold SST, Red, Dark Green/Sodium Heparin, Lavendar/EDTA

Preferred Vol:  3 mL

Minimum Vol:  1 mL

Note: 

Processing Instructions

Reject due to

Spin:  Y

Aliquot:  Y

Temp:  2-8 C

Storage location:  CPA Fridge, FrigF Rack. 

 

Off-site collection:  Spin blood, refrigerate 1 mL serum/plasma in a polypropylene aliquot tube.

Stability

Temperature Time
Room temp  
Refrigerated 7 d
Frozen 6 m

 

Availability

STAT Performed TAT
N* M-F 1-3 d

 

*Available STAT only upon approval of Seattle Children's Hospital Pathologist on call.

 

 

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Chemistry

Location: Chem East

Phone Number: (206) 987-2617

 

 

Reference Range

  AI
Negative < 1.0
Positive > 1.0

 

 

 

 

Methodology

Method:  Immunoassay on Bioplex 2200

Analytical Volume:  0.4 uL serum

Limitations:

 

CPT Codes

CPT CODE(S)
86235 x5