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Test Code FACTOR 5 Factor 5 Activity

Important Note

This test is often confused with a Factor V Leiden (DNA) Test. Method is affected by improper collection, processing or storage, and hemolysis. Patients on hirudin (Refludan) and argatroban (Novastan) may show prolonged clotting times due to the direct thrombin inhibition. This may cause an under-estimation of the factor concentrations.

Clinical System Name

Coagulation Factor 5 Activity

Synonyms

Coagulation Factor 5 Activity
Coagulation Factor V Activity

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (3) 1.8 mL or (1) 2.7 mL

Minimum Vol:  (2) 1.8 mL

 

Note: Preferred method of collection is venipuncture with vacuum fill.  Test results are affected by incorrect blood volume.  Use of a Vascular Access Device for the  collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the  Lt Blue/Citrate tube by use of a  blood transfer device to ensure proper fill. 

Specimen should be centrifuged within 1 hour of collection.

Processing Instructions

Reject due to: Clotted, Hemolyzed, Insufficient quantity, and  Improper collection.

Spin: N

Aliquot: N

Temp: RT

Storage location:  Core 6 Freezer  ( -70 C) Send Out rack.

 

Note:  Deliver blood to Core Coag bench.  Coag Technologist will process. Specimen should be centrifuged within  1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots  0.6 mL, affix large label and store in -70 freezer, Send Out rack.

 

Off-site collection:  Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
Citrated platelet-poor plasma Room temp N
  Refrigerated N
  Frozen Y

 

Availability

STAT Performed TAT
N  M - F 1 - 3 d

 

Performing Laboratory

Harborview Medical Center

Department

Department:  

Harborview Medical Center

Room GWH47; 523 9th Ave
Seattle, WA 98104
 

Phone Number: (206) 744-3451

 

 

Reference Range

 

50 - 150% Activity

 

 

Methodology

Method: Clot based

Analytical Volume: None specified

Limitations:

CPT Codes

85220

Send Out Instructions

 

Reference Test Name: Factor V Activity
Reference Test Number: F5
Instructions: Ship frozen on dry ice. Send out Mon - Fri with the UW/HMC courier. Keep a copy of the Batch Sheet for our records.