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Test Code G6PD QUANT Glucose-6-Phosphate Dehydrogenase (G-6-PD), Quantitative, Erythrocytes

Reporting Name


Useful For

Evaluation of individuals with Coombs-negative nonspherocytic hemolytic anemia


Rapid testing to assess glucose-6-phosphate dehydrogenase (G6PD) enzyme capacity prior to Rasburicase therapy

Specimen Required


Preferred: Yellow top (ACD solution B)

Acceptable: EDTA

Specimen Volume: 6 mL

Collection Instructions: Do not transfer blood to other containers.

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood ACD-B Refrigerated 20 days

Day(s) and Time(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Method Name

Kinetic Spectrophotometry (KS)

Method Description

Glucose-6-phosphate dehydrogenase (G6PD) in a hemolysate catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconate. Concomitantly, nicotinamide adenine dinucleotide phosphate (NADP) is changed to its reduced form (NADPH), a reaction measured spectrophotometrically.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. Third edition. New York, Grune and Stratton, 1984, pp 68-71)

Reference Values

≥12 months: 8.8-13.4 U/g Hb

Reference values have not been established for patients who are <12 months of age.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
G6PD G-6-PD, QN, RBC 32546-4


Result ID Test Result Name Result LOINC Value
G6PD_ G-6-PD, QN, RBC 32546-4

Testing Algorithm

The following algorithms are available in Special Instructions:

-G6PD Genotyping Algorithm for Therapeutic Drug Recommendation

-Newborn Screen Follow-up for Glucose-6-Phosphate Dehydrogenase (G-6-PD) Deficiency


For more information, see Newborn Screening Act Sheet Glucose-6-Phosphate Dehydrogenase Deficiency in Special Instructions.

Reject Due To


Mild OK; Gross reject








If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (