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Test Code GC PCR Neisseria gonorrhoeae PCR

Biohazard Infectious

Synonyms

GC by Amplified Nucleic Acid Detection

CT NG

GC

GC Screen

 

Clincal System Name

Neisseria gonorrhoeae PCR

Description

This test includes nucleic acid amplification for Neisseria gonorrhoeae with amplified probe technique. It is the recommended testing platform for Neisseria gonorrhoeae because of the rapid turn around time and test sensitivity.

 

If actual culture is necessary in order to isolate the organism for sensitivity testing or forensic investication, please collect swab for Genital Culture.

Sample Requirements

Specimen: Dirty Catch Urine, Endocervical Swab, Vaginal Swab

Container:

  • Sterile Container for Urines
  • CT/NG Swab Kit (a.k.a. Xpert CT/NG Vaginal/Endocervical Specimen Collection Kit) for Vaginal and Endocervical sites
  • Aptima Vgainal Collection kit (if sample is bloody).

Preferred Vol: 10mL for Urine

Minimum Vol: 7.0mL for Urine

 

Please consult the Photo Swab Guide to determine which swab to collect.

 

First-catch urine or dirty catch urine with no genital cleaning (NOT mid-stream clean catch) is required. If the patient has submitted a clean catch sample, a dirty catch sample can be submitted on the following day.

 

Clean catch urines are unacceptable for testing and will be REJECTED. Bloody swabs/urine samples will be sent to University of Washington for testing.  Send bloody urine to lab in sterile container.  Collect bloody Vaginal or Endocervical samples with Gen-Probe APTIMA collection kit.

 

Samples received in Aptima collection kits will be forwarded to the University of Washington for testing.

 

This test is validated for urine, endocervical, and vaginal specimens only.  Other specimen sites such as eye, throat, urethral and anal will be accepted but are not optimal for this assay. The preferred collection kit is the Xpert CT/NG (Vaginal/Endocervical) Swab Kit.  UTM swabs are acceptable but not optimal. 

 

Processing Instructions

CPA: Send sample to Microbiology for processing. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

Seattle Campus collection:  Please consult the Photo Swab Guide to determine which swab to collect. 

 

Regional Clinic collection:  Please consult the Photo Swab Guide to determine which swab to collect. Send to SCH Main Campus for testing. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.

 

Stability

Urine
Temp Time
Room temp 24 hours
Refrigerated 1 week
Frozen N

 

Vaginal/Endocervical Swabs in Xpert tranport tube (pink cap)
Temp Time
Room temp 60 d
Refrigerated 60 d
Frozen N

 

Availability

Procedure Performed Turn Around Time (TAT)
In House PCR 24/7

3 - 5 hours

APTIMA PCR (for bloody samples, sent to UW for testing) 24/7 3-5 days

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Microbiology Laboratory

CPT Codes

87591

Reporting Information

Final Report: 3 - 5 hours

 

Notifiable Result

See Link below for Notifiable Conditions for WA state labs:

http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx

 

See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:

http://www.doh.wa.gov/Portals/1/Documents/1200/phsd-LHJ.pdf