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Test Code GENT ; GENT TR ; GENT PK Gentamicin

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

 

Only Random Level is available stat.

Clinical System Name

Gentamicin (Random, Peak, Trough)

Sample Requirements

Specimen: Whole Blood

Container(s): Gold SST, Dark Green/Sodium Heparin, Red, Lavender/EDTA, Gold Microtainer, Lt. Green/Li Heparin Microtainer, or Lavender/EDTA microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note:  Note on the requisition whether the specimen is peak, trough or random. Draw peak level ½ hour after the end of a ½ hour infusion or 1 hour post IM injection. Draw trough level ½ hour before the next dose. Only Random level is available stat.

 

Processing Instructions

Reject due to: Age of specimen

Spin: Y

Aliquot:  Y

Temp: 2-8oC

Storage location:  Core 5 Chemistry Refrigerator

 

Off-site collection:  Note on the requisition whether the specimen is peak, trough or random. Draw peak level ½ hour after the end of a ½ hour infusion or 1 hour post IM injection. Draw trough level ½ hour before the next dose. Only Random Level is available stat.

Stability

 

Specimen Type Temperature Time
Serum or Plasma RT

2 H

Serum or Plasma 2-8oC

 7 D

Serum or Plasma -20oC or -70oC

14 D

 

Availability

STAT Performed TAT
 Y (Random Level Only) 24/7 1 H

 

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Core Chemistry

Phone Number: 206-987-2617

Reference Range

 

All Ages mcg/mL
Peak (Therapeutic) 4 - 12
Trough 0 - 2
Toxic

≥ 12

   
Neonatal Levels and Suggested Dosing  
Trough level taken at 24 hours (mcg/mL) Suggested dosing interval (hours)
< 1 24
1.1 to 2.3 36
2.4 to 3.2 48

 

Methodology

Method:  Two Point Rate method by Vitros 4600

Analytical Volume:  0.02 mL + 0.03 mL dead space

CPT Codes

80170

Critical Values

> 12 mcg/mL