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Test Code HCG Preg HCG Preg

Clinical System Name

HCG, Serum Pregnancy Test


The serum hCG test is a rapid immunoassay for the qualitative detection of human chorionic gonadotropin (hCG) in urine or serum as an aid in the early determination of pregnancy. hCG is a glycoprotein hormone secreted by the developing placenta. After fertilization, the concentration of hCG rapidly rises in both the urine and serum of pregnant women, making the detection of hCG in these fluids an excellent marker for confirming pregnancy. The test utilizes monoclonal and polyclonal antibodies to hCG.

Sample Requirements

Specimen: Whole Blood

Container(s):  Gold SST, Red

Preferred Vol:  3.0 mL

Minimum Vol:  1.0 mL


Notes: Method is affected by pregnancy status and certain tumors. Plasma is unacceptable specimen type.

Indeterminate results are reflexed to UW Laboratory stat for HCG Quant (R/O Preg) testing.

Processing Instructions

Reject due to:  Insufficient Quantity, Plasma specimen

Spin: Y

Aliquot: Y

Temp:  2-8oC

Storage location:  Deliver specimen to Core Lab Urinalysis Bench. Sample will be stored in Core 5 Chemistry Refrigerator.


Off-site collection: Specify: "HCG-R/O Preg." Spin blood, separate serum from cells. Serum May be stored at 2-8 C for 48 hrs. Serum may be frozen and thawed ONCE prior to testing.



Specimen Type Temperature Time
Serum RT

8 hrs.

Serum 2-8oC

48 hrs.

Serum -20oC or -70oC

6 mo.



STAT Performed TAT
Y 24/7 Hospital Lab 1 hr
Y 24/7 Reflexed indeterminate results 2 hr
Y South and Bellevue Labs indeterminate results 4 hr


Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617



Reference Range

Pregnancy Confirmed 10 mIU mL



Method:  Immunochromatographic method for qualitative detection


Analytical Volume:  0.135 mL serum