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Test Code HIPA Heparin Induced Platelet Ab

Clinical System Name

Heparin Induced Platelet Ab

Synonyms

Heparin Induced Ab

HIPA
HIT Antibody

HIT Elisa

Heparin Induced Antibody

Heparin Induced Thrombocytopenia

Heparin-PF4 Antibody

PF4 Heparin Antibody

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: 2.7 mL

Minimum Vol: 1.8 mL

 

Note: Do not use blue band on tube label for correct fill volume. Test results are affected by incorrect blood volume. Preferred method of collection is venipuncture with vacuum fill. Vascular Access Device (VAD) is not the preferred method of collection for coag testing. If this method is used, it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Call Coagulation Lab with questions (206) 987-2561. Specimen must be processed within 6 hours of collection.

Processing Instructions

Reject due to

Spin: Y

Aliquot: Y

Temp: -70 C

Storage location: Core 6 Freezer ( -70 C) Send Out rack.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process. Specimen should be centrifuged within 1hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube and make 2 aliquots 0.6 mL, affix large label and store in -70 freezer, Send Out rack.

 

Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a VAD for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.

Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze 2 aliquots of 0.6 mL plasma in plastic tubes at -70C.

Ship frozen on dry ice.

Stability

Specimen Type Temperature Time
  Room temp

 

  Refrigerated  
  Frozen

 

 

Availability

STAT Performed TAT
Y, with UW Lab Med Resident approval

Daily

 1 - 3 d

 

Note: Samples received before noon run the same day.

Performing Laboratory

University of Washington

Department of Laboratory Medicine

1959 NE Pacific St, NW220
Seattle, WA 98195
 

Phone Number: (206) 598-6066

Department

Department: Send Outs
 

Phone Number: (206) 987-2563

 

 

 

Reference Range

 

Normal Value Critical Value Strong Positive
Negative Positive Elevated thrombotic risk


 

 

 

Methodology

Method: EIA

Analytical Volume: 0.5 mL plasma

Limitations: Method is affected by hemolysis and improper specimen collection/processing.

CPT Codes

86022

Send Out Instructions

 

Reference Test Name:

Heparin Induced Platelet Ab

Reference Test Number:

HIPAB

Instructions:

Ship frozen, M - F with UW courier. For STAT samples, ship on dry ice via Delivery Express courier.