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Test Code HLA ABC HLA ABC by Flow Cytometry

Important Note

Note: limited specimen stability - see Sample Requirements below - do NOT refrigerate.

Children's Outpatient Blood Draw areas and Community Services - order "T&B Community Service" (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.

For more information call Cell Markers (206) 987-2560.

Additional Codes

Panels can be ordered alone, or in combination with other T and B Panels - duplicate tests will not be charged.

Clinical System Name

HLA ABC by Flow Cytometry

Synonyms

HLA Class I by Flow Cytometry

Description

This assay is typically used in the evaluation of primary immunodeficiency, specifically in the setting of possible Bare Lymphocyte Syndrome, in which there is an absence or decrease in the expression of MHC 1 (HLA-ABC) on peripheral white blood cells.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; Dk Green/Heparin also acceptable from referring labs if CBC & differential results submitted

Preferred Vol:  2-3 mL

Minimum Vol:  2 mL; no microtainers

 

Note:  Specimens received after 1430 will be held and testing will be performed the next working day. 

 

 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue)
M-Th collect any time collect any time
Friday collect any time ok if received at SCH main lab same day, if not do not collect
Saturday ok if collected before 12 noon do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon
Sunday ok if collected after 12 noon (to be tested next morning)

ok if collected after 12 noon (to be tested next morning)

Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

 

Children's Outpatient Blood Draw areas and Community Services - order "T&B Community Services" (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.

Processing Instructions

Reject due to:  clotted specimen, Microtainers, Refrigerated

Spin: N

Aliquot: N

Temp:  RT - DO NOT REFRIGERATE

Note:  Have Core Lab run specimen through hematology analyzer and send a copy of the results and copy of community services requisition (if applicable) with specimen to Cell Markers.

Storage location: Days:  Transport specimen, copy of community services requisition (if applicable), and labels to 10th floor Cell Markers (station #280).    Eves/Nights:  Store specimen, copy of community services requisition (if applicable), and labels in the Cell Markers RT box in CPA.

 

Off-site collection:  Keep whole blood at room temperature. Referring labs may send CBC w/differential results from the same collection along with the sample.

Stability

Temperature Time
Room temp EDTA - 30 hours; Heparin - 48 hours
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
N M-F, Sa (0730-1200) 2-3 days

Note: limited specimen stability - see Sample Requirements

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

Reference Range

Reference values accompany patient report.
 

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on patient's white count and testing requested

Limitations:

CPT Codes

contact Laboratory Client Services at (206)-987-2617