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Test Code HLA B27 HLA B27 Typing

Clinical System Name

HLA B27

Synonyms

Tissue Typing – HLA B 27

Description

This qualitative assay is used to identify the B27 Human Leukocyte Antigen.  The presence of the HLA-B27 antigen in a patient with symptoms has been found to be highly associated with Ankylosing Spondylitis and related disorders.

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 1 mL

Minimum Vol: 0.5 mL

 

Note: Confirmatory testing of equivocal results is available at Bloodworks Northwest. If confirmatory testing is requested, please place a CIS add-on for a MISC test. In the order comments, please enter "HLA B27 Confirmatory to BWNW". CPA: please add testing to original HLA B27 container (Lavender/EDTA) found in Cell Markers and deliver to Send Outs room temp rack. Sample stable for one week at room temperature.

 

Processing Instructions

Reject due to: n/a - send to lab

Spin: N

Aliquot: N

Temp: RT

Storage location: Days: Transport specimen and labels to 10th floor Cell Markers (station #280). Eves/Nights:  Store specimen and labels in the Cell Markers RT box in CPA.

 

Off-site collection: Keep whole blood at room temperature and transport to Seattle Childrens.

Stability

Temperature Time
Room temp 72 hours
Refrigerated

N

Frozen N

 

Availability

STAT Performed TAT
N collected daily; performed M-Sat 2-3 days

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department: Cell Markers

Phone Number: 206-987-2560

 

Reference Range

 

Negative

 

Found in Caucasians 6 - 8%, African-Americans 3 - 4%, and Asians 1%.

Methodology

Method:  Flow Cytometry

Analytical Volume:  50 microL whole blood

Limitations:  Method is affected by age of sample, heat and cold. Not affected by heparin.

CPT Codes

CPT CODE
86812